Oxidative damage to DNA in lymphocytes from AD patients
Oxidative damage to DNA in lymphocytes from AD patients
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DOI：
10.1212/wnl.51.4.1014
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发表时间：
1998-10-01
期刊：
影响因子：
9.9
通讯作者：
Senin, U
中科院分区：
文献类型：
--
作者：
Mecocci, P;Polidori, MC;Senin, U
目的：多项研究显示AD患者周围细胞结构和功能发生改变。本研究的目的是评估AD淋巴细胞的氧化应激。背景：文献支持活性氧在阿尔茨海默病发病机制中的作用，因为在阿尔茨海默病大脑中检测到几种氧化损伤标志物。方法：采用高压液相色谱电化学检测法测定AD患者和健康老年人基础状态和急性过氧化氢氧化应激后淋巴细胞中8-羟基-2′-脱氧鸟苷（8OHdG）的DNA氧化应激标志物。结果：AD患者淋巴细胞中8OHdG浓度明显高于对照组。在后一组中，8OHdG随着年龄的增长而逐渐增加。急性氧化应激后，AD患者和对照组之间形成的8OHdG水平没有差异。结论：我们的研究结果支持AD受氧化应激的影响，氧化应激不仅在大脑中检测到，而且在外周细胞中检测到；氧化机制可能与AD的发病机制有关。需要其他神经退行性疾病的进一步研究来评估这些发现。
Objective: Several studies show structural and functional alterations in peripheral cells in AD. The purpose of this study was to evaluate oxidative stress in AD lymphocytes. Background: The literature supports the role of reactive oxygen species in the pathogenesis of AD because several markers of oxidative damage have been detected in AD brain. Methods: 8-hydroxy-2'-deoxyguanosine (8OHdG), a marker of oxidative stress in DNA, was measured in lymphocytes of AD patients and healthy aged controls with high-pressure liquid chromatography with electrochemical detection, both at basal condition and after acute oxidative stress with hydrogen peroxide. Results: A significantly higher concentration of 8OHdG in lymphocytes occurred in AD patients compared with controls. In this latter group, 8OHdG increased progressively with age. After acute oxidative stress, levels of formed 8OHdG did not differ between AD patients and controls. Conclusions: Our results support that AD is affected by oxidative stress, detectable not only in the brain but also in peripheral cells; oxidative mechanisms may contribute to the pathogenesis of AD. Additional studies in other neurodegenerative diseases are needed to evaluate these findings.