Layer-Specific Targeting of Direction-Selective Neurons in the Zebrafish Optic Tectum
Layer-Specific Targeting of Direction-Selective Neurons in the Zebrafish Optic Tectum
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DOI：
10.1016/j.neuron.2012.12.003
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发表时间：
2012-12-20
期刊：
影响因子：
16.2
通讯作者：
Bollmann, Johann H.
中科院分区：
文献类型：
--
作者：
Gabriel, Jens P.;Trivedi, Chintan A.;Bollmann, Johann H.
方向选择性（DS）是视觉系统中一个重要的神经元特性，但DS如何在视网膜外产生仍然存在争议。在这里，我们报告的首选方向（PD）和DS细胞在视顶盖的形态之间的密切对应关系。表达遗传编码的Ca2+指示剂GCaMP3的细胞中的Ca2+成像和多光子靶向膜片钳记录使我们能够比较单个神经元的结构和功能。不同调谐的细胞类型的乔木表现出刻板的形状和层状轮廓内的顶盖神经节的差异。兴奋性突触输入的定向调谐和匹配的PD在这些细胞中的尖峰输出，而抑制性输入的选择性非首选方向。传入轴突的功能性Ca2+成像显示DS突触前活动的匹配层状分布。因此，不同的方向表示在不同的层，这表明一个简单的机制，如何顶盖神经元获得定向调谐在一个新生的电路。
Direction selectivity (DS) is an important neuronal property in the visual system, but how DS is generated beyond the retina remains controversial. Here, we report a close correspondence between the preferred direction (PD) and the morphology of DS cells in the optic tectum. Ca2+ imaging in cells expressing the genetically encoded Ca2+ indicator GCaMP3 and multiphoton-targeted patch-clamp recordings allowed us to compare structure and function in single neurons. The arbors of differently tuned cell types showed stereotypic differences in shape and laminar profile within the tectal neuropil. Excitatory synaptic inputs were directionally tuned and matched the PD of spike output in these cells, while inhibitory inputs were selective for nonpreferred directions. Functional Ca2+ imaging in afferent axons showed a matching laminar distribution of DS presynaptic activity. Hence, different directions are represented in different layers, which suggests a simple mechanism for how tectal neurons acquire directional tuning in a nascent circuit.