HEART EXCITATORY AND INHIBITORY SUBSTANCES IN MOLLUSCAN GANGLIA
HEART EXCITATORY AND INHIBITORY SUBSTANCES IN MOLLUSCAN GANGLIA
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DOI：
10.1016/0010-406x(67)90775-x
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发表时间：
1967-01-01
期刊：
影响因子：
--
通讯作者：
WELSH, JH
中科院分区：
文献类型：
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作者：
FRONTALI, N;WILLIAMS, L;WELSH, JH
已知来自某些软体动物的神经和心脏组织提取物除了含有5 HT之外还含有心脏兴奋物质。我们用Sephadex G-15柱对该物质进行了分析。在用1-甲基-麦角酸丁酰胺和Mytelase处理的蛤蛤M.紫藤产生4个心脏兴奋活性峰（峰A、B、C、D）。蛤心提取物仅产生峰A、B和C。来自蜗牛Busycon canaliculatum神经节的提取物产生峰C和D。通过用蛋白水解酶处理，蛤或蜗牛的峰A、B和C完全失活;峰D未失活。
Nerve and heart tissue extracts from some molluscs are known to contain heart excitatory material in addition to 5HT. We have used Sephadex G-15 columns to analyze this material. Eluted fractions were assayed on hearts of the clam Mercenaria mercenaria treated with 1-methyl-lysergic acid butanolamide and with Mytelase in order to counteract 5-hydroxytryptamine and acetylcholine. Extracts from ganglia of M. mercenaria gave rise to 4 peaks of cardioexcitatory activity (peaks A, B, C, D). Extracts from clam hearts gave rise only to peaks A, B and C. Extracts from ganglia of the snail Busycon canaliculatum gave rise to peaks C and D. Peaks A, B and C from either the clam or the snail were completely inactivated by treatment with proteolytic enzymes; peak D was not inactivated.