Organization and transcriptional regulation of myo-inositol operon in Clostridium perfringens
Organization and transcriptional regulation of myo-inositol operon in Clostridium perfringens
复制标题
DOI：
10.1016/j.femsle.2004.04.047
复制
发表时间：
2004-06-15
影响因子：
2.1
通讯作者：
Shimizu, T
中科院分区：
文献类型：
--
作者：
Kawsar, HI;Ohtani, K;Shimizu, T
产气荚膜梭菌13株的肌醇操纵子由13个基因组成，其上游有一个不同向的调控基因iolR。转录分析显示该操纵子有三种不同的转录本，长度分别为15.6、4.6和2.0kb。iolR突变研究表明，IolR在转录水平上是该操纵子的负调控因子。所有转录本都以剂量和时间依赖的方式被肌醇诱导。葡萄糖抑制肌醇操纵子所有转录本的表达。我们还发现，无论是否存在肌醇，两组分VirR/VirS系统都对该操纵子进行正调控。这项研究表明，全局调控的VirR/VirS系统除了控制产气荚膜梭菌13株的毒力基因外，还控制与能量产生相关的基因（如肌醇操纵子）的表达。（C）2004欧洲微生物学会联合会。由爱思唯尔出版社出版。保留所有权利。
myo-Inositol operon of Clostridium perfringens strain 13 consists of 13 genes with an upstream divergent regulator, iolR. Transcriptional analysis showed three separate transcripts for the operon of 15.6, 4.6 and 2.0 kb in length. iolR mutation studies showed that IolR is a negative regulator of the operon at transcriptional level. All the transcripts were induced by myo-Inositol in dose- and time-dependent manner. Glucose repressed the expression of all the transcripts of myo-Inositol operon. We also found that the operon was positively regulated by the two-component VirR/VirS system both in the presence and absence of myo-Inositol. This study shows that the global regulatory VirR/VirS system controls the expression of genes related to energy production (e.g. myo-Inositol operon) in addition to the virulence genes of C. perfringens strain 13. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.