Identification and Characterization of Leucocyclicin Q, a Novel Cyclic Bacteriocin Produced by Leuconostoc mesenteroides TK41401
Identification and Characterization of Leucocyclicin Q, a Novel Cyclic Bacteriocin Produced by Leuconostoc mesenteroides TK41401
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DOI：
10.1128/aem.06348-11
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发表时间：
2011-11-01
影响因子：
4.4
通讯作者：
Sonomoto, Kenji
中科院分区：
文献类型：
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作者：
Masuda, Yoshimitsu;Ono, Hiroshi;Sonomoto, Kenji
从日本泡菜中分离到的肠系膜明串珠菌TK41401的培养上清液对多种细菌属均有抑菌活性，尤其对泡菜的主要污染物凝结芽孢杆菌具有较强的抑菌活性。通过三步层析纯化得到一种抗菌肽，用电喷雾电离飞行时间质谱仪(ESI-TOF MS)测定其相对分子质量为6115.59Da。通过氨基酸和DNA测序确定了该多肽的一级结构，这些分析表明它被翻译成一个63个残基的前体。该前体与乳球菌产生的环状细菌素乳环素Q的前体有很高的相似性。菌株Qu-12环化后的分子量与ESI-TOF MS测定值一致，推测与乳环素Q(L3~W63)的环化过程相同，证明该多肽是一种新的环状细菌素，命名为白环素Q。该细菌素的抗菌谱与乳环素Q明显不同，尽管它们的一级结构非常相似。这是首次报道由明串珠菌属菌株产生的环状细菌素。
The culture supernatant of Leuconostoc mesenteroides TK41401, isolated from Japanese pickles, possessed antimicrobial activity against broad range of a bacterial genera and particularly strong activity against Bacillus coagulans, the major contaminant of pickles. An antimicrobial peptide was purified in three chromatographic steps, and its molecular mass was determined to be 6,115.59 Da by electrospray ionization time-of-flight mass spectrometry (ESI-TOF MS). The primary structure of this peptide was determined by amino acid and DNA sequencing, and these analyses revealed that it was translated as a 63-residue precursor. This precursor showed high similarity to the precursor of lactocyclicin Q, a cyclic bacteriocin produced by Lactococcus sp. strain QU 12. The molecular weight calculated after cyclization, which was presumed to involve the same process as in lactocyclicin Q (between L3 and W63), agreed with that estimated by ESI-TOF MS. This peptide was proved to be a novel cyclic bacteriocin, and it was termed leucocyclicin Q. The antimicrobial spectrum of this bacteriocin clearly differed from that of lactocyclicin Q, even though their primary structures were quite similar. This is the first report of a cyclic bacteriocin produced by a strain of the genus Leuconostoc.