Spatial patterns of iron- and methane-oxidizing bacterial communities in an irregularly flooded, riparian wetland.
Spatial patterns of iron- and methane-oxidizing bacterial communities in an irregularly flooded, riparian wetland.
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DOI：
10.3389/fmicb.2012.00064
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发表时间：
2012
影响因子：
5.2
通讯作者：
Bodelier PL
中科院分区：
文献类型：
--
作者：
Wang J;Krause S;Muyzer G;Meima-Franke M;Laanbroek HJ;Bodelier PL
铁和甲烷的循环是湿地中的重要过程，其中一个与植物生长有关，另一个与温室气体排放有关。与酸性生境相比，关于微生物在中性pH值下氧化亚铁的生态信息很少。后者主要是由于缺乏分离的代表性和分子检测技术。最近，我们发展了PCR-DGGE和qPCR方法来检测和计数与加里奥氏菌相关的中性氧化铁(Ga-FeOB)，从而能够评估各种生态系统中控制物理和生物因素。在这项研究中，我们调查了河岸湿地中Ga-FeOB与甲烷氧化细菌(MOB)共生的空间分布。土壤样本是在不同的空间尺度(从米到厘米)上采集的，代表了一种水文梯度。用聚合酶链式反应-差示扫描量热法分析了Ga-FeOB的多样性，用定量聚合酶链式反应分析了Ga-FeOB和MOB的丰度。应用地统计学方法对两组的空间分布进行可视化。Ga-FeOB和MOB的空间分布和丰度与土壤含水率所表示的水文梯度有明显的相关性。GA-FeOB的数量超过了暴徒亚群，这表明在这片洪泛平原上，他们的竞争力或Fe2+高于CH4氧化的普遍程度。
Iron- and methane-cycling are important processes in wetlands with one connected to plant growth and the other to greenhouse gas emission, respectively. In contrast to acidic habitats, there is scarce information on the ecology of microbes oxidizing ferrous iron at circumneutral pH. The latter is mainly due to the lack of isolated representatives and molecular detection techniques. Recently, we developed PCR–DGGE and qPCR assays to detect and enumerate Gallionella-related neutrophilic iron-oxidizers (Ga-FeOB) enabling the assessment of controlling physical as well as biological factors in various ecosystems. In this study, we investigated the spatial distribution of Ga-FeOB in co-occurrence with methane-oxidizing bacteria (MOB) in a riparian wetland. Soil samples were collected at different spatial scales (ranging from meters to centimeters) representing a hydrological gradient. The diversity of Ga-FeOB was assessed using PCR–DGGE and the abundance of both Ga-FeOB and MOB by qPCR. Geostatistical methods were applied to visualize the spatial distribution of both groups. Spatial distribution as well as abundance of Ga-FeOB and MOB was clearly correlated to the hydrological gradient as expressed in moisture content of the soil. Ga-FeOB outnumbered the MOB subgroups suggesting their competitiveness or the prevalence of Fe2+ over CH4 oxidation in this floodplain.
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影响因子：
4.4
作者：
Emerson, D;Moyer, C
通讯作者：
Moyer, C
DOI：
10.1073/pnas.0808254105
发表时间：
2008-11-18
影响因子：
11.1
作者：
Hartman, Wyatt H.;Richardson, Curtis J.;Bruland, Gregory L.
通讯作者：
Bruland, Gregory L.
影响因子：
4.4
作者：
Bloethe, Marco;Roden, Eric E.
通讯作者：
Roden, Eric E.
影响因子：
9.7
作者：
Gebert, Julia;Groengroeft, Alexander;Pfeiffer, Eva-Maria
通讯作者：
Pfeiffer, Eva-Maria
影响因子：
3.7
作者：
Fleming EJ;Langdon AE;Martinez-Garcia M;Stepanauskas R;Poulton NJ;Masland ED;Emerson D
通讯作者：
Emerson D