Vacuoles induced by Helicobacter pylori toxin contain both late endosomal and lysosomal markers
Vacuoles induced by Helicobacter pylori toxin contain both late endosomal and lysosomal markers
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DOI：
10.1074/jbc.272.40.25339
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发表时间：
1997-10-03
影响因子：
4.8
通讯作者：
Montecucco, C
中科院分区：
文献类型：
--
作者：
Molinari, M;Galii, C;Montecucco, C
幽门螺杆菌释放的空泡毒素（VacA）对哺乳动物细胞的毒性作用导致形成含有空泡ATP酶质子泵和Rab 7（一种晚期内体标记物）的大酸性空泡。在这里，我们描述了一种新的亚细胞分馏程序，我们表明，纳摩尔浓度的VacA诱导溶酶体膜糖蛋白的内吞隔室之间的明确重新分配。这种再分配是VacA细胞中毒过程中的早期事件，并先于宏观空泡的形成。阳离子非依赖性甘露糖6-P受体的情况下，Rab 7和溶酶体膜蛋白的存在下，在新形成的隔室表明，空泡毒素诱导后内体混合隔室的积累，呈现晚期内体和溶酶体的功能。
Intoxication of mammalian cells with the vacuolating toxin (VacA) released by Helicobacter pylori causes the formation of large acidic vacuoles containing the vacuolar ATPase proton pump and Rab7, a late endosome marker. Here, we describe a novel subcellular fractionation procedure, and we show that nanomolar concentrations of VacA induce a clear redistribution of lysosomal membrane glycoproteins among endocytic compartments. This redistribution is an early event in the process of cellular intoxication by VacA and precedes the formation of macroscopic vacuoles. The absence of the cation independent mannose 6-P receptor and the presence of Rab7 and of lysosomal membrane proteins in the newly formed compartment suggest that the vacuolating toxin induces the accumulation of a post-endosomal hybrid compartment presenting both late endosomal and lysosomal features.