A novel C-type lectin from Eriocheir sinensis functions as a pattern recognition receptor with antibacterial activity
A novel C-type lectin from Eriocheir sinensis functions as a pattern recognition receptor with antibacterial activity
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中华绒螯蟹中一种新型C型凝集素作为具有抗菌活性的模式识别受体
DOI：
10.1016/j.fsi.2013.08.021
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发表时间：
2013-11-01
影响因子：
4.7
通讯作者：
Wang, Qun
中科院分区：
文献类型：
--
作者：
Guo, Xiao-Nv;Jin, Xing-Kun;Wang, Qun
c型凝集素（ctl）作为模式识别受体（PRRs），在先天免疫中识别和清除病原体发挥着重要作用。本研究从中华绒螯蟹（Eriocheir sinensis）中鉴定出一个新的CTL （EsLecD）。EsLecD全长cDNA的克隆是基于从肝胰cDNA文库中分离的初始表达序列标签（ESTs）。EsLecD全长686 bp，开放阅读框468 bp，编码155个氨基酸残基的蛋白，包括一个n端信号肽和一个碳水化合物识别结构域（CRD）。通过定量RT-PCR分析，EsLecD转录物主要在肝胰脏中检测到，而在其他组织中很少检测到，并且在脂多糖免疫攻击后，EsLecD转录物在肝胰脏中显著上调。重组EsLecD蛋白（rEsLecD）显示出与所有被测微生物（包括细菌和酵母）结合的能力。同时，钙显著提高了rEsLecD对微生物的结合亲和力，但不是必需的。rEsLecD的结合诱导了微生物病原体的聚集。此外，rEsLecD能够抑制微生物的生长，甚至直接杀死细菌。有趣的是，rEsLecD可以刺激体外细胞包封。综上所述，本研究结果提示EsLecD作为一种抗菌PRR参与无脊椎动物先天免疫。(C) 2013 Elsevier Ltd.版权所有。
As pattern recognition receptors (PRRs), C-type lectins (CTLs) play significant roles in recognizing and eliminating pathogens in innate immunity. In this study, a novel CTL (EsLecD) was identified from the crustacean Eriocheir sinensis. The cloning of full-length EsLecD cDNA was based on the initial expressed sequence tags (ESTs) isolated from a hepatopancreatic cDNA library. The full-length EsLecD cDNA of 686 bp with an open reading frame of 468 bp encodes a putative protein of 155 aa residues, including an N-terminal signal peptide and a single carbohydrate-recognition domain (CRD). By quantitative RT-PCR analysis, the EsLecD transcript was mainly detected in the hepatopancreas but rarely in other tissues, and it was significantly upregulated in the hepatopancreas after immune challenge with lipopolysaccharides. The recombinant EsLecD protein (rEsLecD) exhibited the ability to bind to all tested microorganisms, including bacteria and yeast. Meanwhile, calcium significantly increased the binding affinity of rEsLecD toward microorganisms, but it was not essential. The binding of rEsLecD induced the aggregation of microbial pathogens. Moreover, rEsLecD was capable of inhibiting the growth of microorganisms and even directly killing bacteria. Interestingly, rEsLecD could stimulate cellular encapsulation in vitro. In conclusion, results of this study suggest that EsLecD acts as an antibacterial PRR participating in the innate immunity of invertebrates. (C) 2013 Elsevier Ltd. All rights reserved.