Distinct NMDA and AMPA receptor-mediated responses in mouse and human Cajal-Retzius cells.
Distinct NMDA and AMPA receptor-mediated responses in mouse and human Cajal-Retzius cells.
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小鼠和人类 Cajal-Retzius 细胞中不同的 NMDA 和 AMPA 受体介导的反应。
DOI：
10.1152/jn.2001.86.5.2642
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Yeh,HH
中科院分区：
文献类型：
--
作者：
Lu,SM;Zecevic,N;Yeh,HH
本研究检测了小鼠和人Cajal-Retzius(C-R)细胞对谷氨酸激活的电流反应。从出生后4-6天的小鼠和中期妊娠的人类胎儿的大脑中制备薄层皮质切片。人和小鼠C-R细胞均表现出电压依赖性的谷氨酸诱导的全细胞电流反应，其中包括NMDA受体介导的成分，该成分对NMDA受体拮抗剂2-氨基-5-膦-丙戊酸和异丙苯地尔的阻断具有不同的敏感性。α-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic酸是一种非N-甲基-D-天冬氨酸受体激动剂，能在人细胞上引起电流反应，但在小鼠的C-R细胞上不能引起电流反应。综上所述，我们得出结论，人类C-R细胞在皮质发育的早期就表达NMDA和AMPA类型的谷氨酸受体。相反，小鼠的C-R细胞只表达NMDA型谷氨酸受体。因此，我们证明了C-R细胞对谷氨酸的敏感性依赖于物种，并假设这种差异敏感性可能在一定程度上解释了C-R细胞在皮质发育过程中持续时间的物种依赖差异。
This study examined glutamate-activated current responses of mouse and human Cajal-Retzius (C-R) cells. Thin cortical slices were prepared from the brains of mice 4–6 days after birth and from those of midgestational human fetuses. Both human and mouse C-R cells displayed glutamate-induced whole-cell current responses that were voltage-dependent and included anN-methyl-d-aspartate (NMDA) receptor–mediated component that was differentially sensitive to blockade by the NMDA receptor antagonists 2-amino-5-phosphonovaleric acid and ifenprodil. α-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA), a non-NMDA glutamate receptor agonist, induced current responses in human but not in mouse C-R cells. These results, taken together, lead us to conclude that human C-R cells express both NMDA and AMPA types of glutamate receptors very early during development of the cortex. In contrast, mouse C-R cells express only the NMDA type of glutamate receptor. Thus we demonstrate a species-dependent sensitivity of C-R cells to glutamate and postulate that this differential sensitivity may account in part for a species-dependent difference in the persistence of C-R cells during cortical development.