Rapid isoelectric trapping in a micropreparative-scale multicompartment electrolyzer
Rapid isoelectric trapping in a micropreparative-scale multicompartment electrolyzer
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DOI：
10.1002/elps.200600846
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发表时间：
2007-06-01
期刊：
影响因子：
2.9
通讯作者：
Vigh, Gyula
中科院分区：
文献类型：
--
作者：
Lim, Peniel;North, Robert;Vigh, Gyula
一种称为等电聚焦和捕获膜分离井 (MSWIFT) 的多室电解槽已开发出来，并针对微量制备规模的等电捕获分离进行了测试。在MSWIFT中，从隔室中心到墙壁的热传导路径的长度小于1毫米。隔室由 99.8% 无孔氧化铝制成，具有高导热率和高比热容，即使在高达 5 W 的功率负载下也能实现足够的焦耳散热。任何平行于电场的隔室长度（膜间距离）可选择为 1.5 mm 的倍数，从而使隔室体积约为 60 PL 的倍数。当前版本的 MSWIFT 中最多可轻松组装 20 个（1.5 毫米长）分离隔室。 MSWIFT 已用于样品脱盐、分离小量两性成分（氨基酸、肽和染料）、预分级复杂蛋白质混合物以及富集微量成分；这些分离在 20-60 分钟内完成。
A multicompartmental electrolyzer called membrane-separated wells for isoelectric focusing and trapping (MSWIFT) has been developed and tested for micropreparative-scale isoelectric trapping separations. In the MSWIFT, the length of the heat conduction path from the center of the compartments to the wall is less than 1 mm. The compartments are made from 99.8% nonporous alumina that has a high heat conductivity and a high specific heat capacity, leading to adequate joule heat dissipation even at power loads as high as 5 W The length of any compartment parallel to the electric field (the intermembrane distance) can be selected to be multiples of 1.5 mm, leading to compartment volumes that are multiples of about 60 PL. A maximum of 20 (1.5 mm. long) separation compartments can be readily assembled in the current version of MSWIFT. The MSWIFT has been used to desalt samples, isolate small ampholytic components (amino acids, peptides, and dyes), prefractionate complex protein mixtures and enrich minor components; these separations were achieved in 20-60 min.