Recovery of mycobacteriophages from archival stocks stored for approximately 50 years in Japan.
Recovery of mycobacteriophages from archival stocks stored for approximately 50 years in Japan.
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从日本保存约 50 年的档案库存中回收分枝杆菌噬菌体。
DOI：
10.1007/s00705-018-3788-8
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发表时间：
2018
影响因子：
2.7
通讯作者：
Matsuzaki S.
中科院分区：
文献类型：
--
作者：
Ujihara T;Uchiyama J;Nasukawa T;Ando H;Murakami H;Ohara N;Ogawa M;Yamazaki T;Daibata M;Sakaguchi M;Matsuzaki S.
分枝杆菌噬菌体档案库保存了约10年。日本20-50年。在这项研究中，我们试图从50个档案股票回收分枝杆菌噬菌体，并简要分析了回收的噬菌体。从贮存47-56年的冻干原液中回收了72.2%（13/18）的细菌。此外，12个代表性的回收病毒的分析导致其属于家庭Siphoviridae的分类，其中7个通过聚合酶链反应（PCR）靶向编码卷尺蛋白的基因进行分型。考虑到这些结果，冻干似乎适合于噬菌体档案储存。
Mycobacteriophage archival stocks have been kept for ca. 20–50 years in Japan. In this study, we attempted to recover mycobacteriophages from 50 archival stocks and briefly analyzed the recovered phages. The phages were recovered from 72.2% (13/18) of the lyophilized stocks that had been stored for 47-56 years. Moreover, the analysis of 12 representative recovered phages led to their classification as belonging to the familySiphoviridae, and seven of them were typed by polymerase chain reaction (PCR) targeting the gene that encodes the tape measure protein. Considering these results, lyophilization seems to be suitable for phage archival storage.