Seed-Specific Expression of OsDWF4, a Rate-Limiting Gene Involved in Brassinosteroids Biosynthesis, Improves Both Grain Yield and Quality in Rice
Seed-Specific Expression of OsDWF4, a Rate-Limiting Gene Involved in Brassinosteroids Biosynthesis, Improves Both Grain Yield and Quality in Rice
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OsDWF4（一种参与油菜素类固醇生物合成的限速基因）的种子特异性表达可提高水稻的产量和品质
DOI：
10.1021/acs.jafc.8b00077
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发表时间：
2018-04-18
影响因子：
6.1
通讯作者：
Liu, Qiao-Quan
中科院分区：
文献类型：
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作者：
Li, Qian-Feng;Yu, Jia-Wen;Liu, Qiao-Quan
油菜素类固醇(BRs)是植物特有的必需类固醇激素，调节植物不同的生长发育过程。我们评估了编码BR生物合成限速酶的基因OsDWF4在GT1或Ubi启动子的驱动下对水稻产量和品质的影响，GT1或Ubi启动子分别对应于种子特异表达或结构性表达。一般而言，转OsDWF4基因植株表现出更高的产量和更多的分蘖和更长更重的种子。此外，转基因大米的淀粉理化性质也得到了改善。有趣的是，在不同的促进剂的驱动下，OsDWF4对水稻产量或品质的影响是不同的。PGt1：：OsDWF4系的整体性能优于pUbi：：OsDWF4系。我们的数据不仅证明了OsDWF4过表达对水稻产量和品质的影响，而且也表明种子特异性启动子在BR介导的水稻育种中是一个很好的选择。
Brassinosteroids (BRs) are essential plant-specific steroidal hormones that regulate diverse growth and developmental processes in plants. We evaluated the effects of OsDWF4, a gene that encodes a rate-limiting enzyme in BR biosynthesis, on both rice yield and quality when driven by the Gt1 or Ubi promoter, which correspond to seed-specific or constitutive expression, respectively. Generally, transgenic plants expressing OsDWF4 showed increased grain yield with more tillers and longer and heavier seeds. Moreover, the starch physicochemical properties of the transgenic rice were also improved. Interestingly, OsDWF4 was found to exert different effects on either rice yield or quality when driven by the different promoters. The overall performance of the pGt1::OsDWF4 lines was better than that of the pUbi::OsDWF4 lines. Our data not only demonstrate the effects of OsDWF4 overexpression on both rice yield and quality but also suggest that a seed-specific promoter is a good choice in BR-mediated rice breeding programs.