Subretinal semiconductor microphotodiode array.
Subretinal semiconductor microphotodiode array.
复制标题
视网膜下半导体微光电二极管阵列。
DOI：
10.3928/1542-8877-19980301-10
复制
发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Neal S. Peachey
中科院分区：
文献类型：
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作者：
Gholam A. Peyman;A. Chow;C. Liang;V. Chow;Jay I. Perlman;Neal S. Peachey
背景与目的
目的：研究手术植入视网膜下腔的半导体微光电二极管阵列（SMA）的功能。
材料和方法
通过睫状体平坦部切口和后部视网膜切开术，将阳性-内在层-阴性（PiN）或阴性-内在层-阳性（NiP）SMA手术置入兔视网膜下腔。植入物不需要外部连接电源，并且对可见光和红外（IR）光谱的光敏感; IR刺激用于将植入物介导的反应与天然光感受器的活性隔离。使用刺激器检眼镜对植入物和邻近视网膜区域进行IR刺激，并在术后恢复期记录反应。还在体外评价了SMA反应。这些动物被给予过量致死的麻醉剂，并对视网膜进行组织学检查。
结果
体外植入反应包括电尖峰，随后是小幅度DC偏移，其遵循IR刺激的时间过程，以及刺激偏移处的过冲。放置在视网膜下腔的SMA保持稳定的位置，并在整个术后期间继续发挥功能。在体内记录的SMA反应包括在体外记录中不存在的附加慢波分量。这些反应在动物死亡后恢复到体外构型。在植入物上覆的区域中存在视网膜细胞的显著损失，并且远离植入物和手术部位的视网膜看起来正常。
结论
SMA可以成功植入视网膜下腔，并在延长的时间段内响应于光刺激产生电流。
BACKGROUND AND OBJECTIVE
To examine the function of a semiconductor microphotodiode array (SMA) surgically implanted in the subretinal space.
MATERIALS AND METHODS
Positive-intrinsic layer-negative (PiN) or negative-intrinsic layer-positive (NiP) SMAs were surgically placed into the subretinal space of rabbits through a pars plana incision and a posterior retinotomy. The implants required no external connections for power and were sensitive to light over the visible and infrared (IR) spectrum; IR stimuli were used to isolate implant-mediated responses from the activity of native photoreceptors. A stimulator ophthalmoscope was used to superimpose IR stimuli on the implant and adjacent retinal areas, and responses were recorded during the postoperative recovery period. SMA responses were also evaluated in vitro. The animals were given lethal anesthetic overdoses, and the retinas were examined histologically.
RESULTS
The in vitro implant response consisted of an electrical spike, followed by a small-amplitude DC offset that followed the time course of the IR stimulation, and an overshoot at the stimulus offset. The SMAs placed in the subretinal space retained a stable position and continued to function throughout the postoperative period. The SMA responses recorded in vivo included additional slow-wave components that were absent from the in vitro recordings. These responses reverted to the in vitro configuration following the death of the animal. There was a significant loss of retinal cells in areas overlying the implant, and the retina appeared normal away from the implant and surgical site.
CONCLUSION
SMAs can be successfully implanted into the subretinal space and will generate current in response to light stimulation during an extended period of time.