Mapping of cosmid clones in Huntington's disease region of chromosome 4.
Mapping of cosmid clones in Huntington's disease region of chromosome 4.
复制标题
4 号染色体亨廷顿病区域粘粒克隆的定位。
DOI：
10.1007/bf01233207
复制
发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Youngman,S
中科院分区：
文献类型：
--
作者：
Whaley,WL;Bates,GP;Novelletto,A;Sedlacek,Z;Cheng,S;Romano,D;Ormondroyd,E;Allitto,B;Lin,C;Youngman,S
亨廷顿氏病（HD）与4p16.3的遗传标记密切相关。我们使用区域体细胞杂交图谱分离和绘制了25个cosmids到4p16.3的近端部分和17个cosmids到远端部分。后者通过相对于先前绘制的标记的远程限制性映射来定位。其中一个cosmid L6 （D4S166）跨越了区分近端和远端4p16.3的映射面板中的关键断点。其中4个cosmids远端指向d4s90，即4p上的前一个末端标记，并延伸到端粒75 kb以内。几个在L6和d4s90之间定位的cosmids聚集在一个有许多NotI位点的区域的许多先前分离的克隆附近。Cosmid E4 （D4S168）位于远端4p16.3远端限制性图谱中剩余的一个缺口的近端。虽然用E4进行脉冲场凝胶定位未能将图谱的两个片段连接起来，但通过遗传分析排除了中间间隙作为hdgene的潜在位点。
Huntington's disease (HD) is tightly linked to genetic markers in 4p16.3. We have used a regional somatic cell hybrid mapping panel to isolate and map 25 cosmids to the proximal portion of 4p16.3 and 17 cosmids to the distal portion. The latter were positioned by long-range restriction mapping relative to previously mapped markers. One cosmid, L6 (D4S166), spans the critical breakpoint in the mapping panel that distinguishes proximal and distal 4p16.3. Four of the cosmids mapped distal toD4S90, the previous terminal marker on 4p, and stretched to within 75 kb of the telomere. Several of the cosmids that mapped between L6 andD4S90were clustered near a number of previously isolated clones in a region with many NotI sites. Cosmid E4 (D4S168) was localized immediately proximal to the one remaining gap in the long-range restriction map of distal 4p16.3. Although pulsed field gel mapping with E4 failed to link the two segments of the map, the intervening gap was excluded as a potential site for theHDgene by genetic analysis.