Recombination in feline lentiviral genomes during experimental cross-species infection.
Recombination in feline lentiviral genomes during experimental cross-species infection.
复制标题
实验性跨物种感染期间猫慢病毒基因组的重组。
DOI：
10.1016/j.virol.2006.08.026
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发表时间：
2007
期刊：
影响因子：
3.7
通讯作者：
Rodrigo,Allen
中科院分区：
文献类型：
--
作者：
Poss,Mary;Idoine,Adam;Ross,HowardA;Terwee,JulieA;VandeWoude,Sue;Rodrigo,Allen
家猫发展为无症状的、生产性的猫免疫缺陷病毒（PLV）感染，该病毒来源于自然感染的美洲狮（P. concolor）。我们先前证明，在这种跨物种感染期间，PLV基因组中存在广泛的G到A置换，这是宿主胞苷脱氨酶编辑的特征，并且对逆转录酶进行正选择。在这项研究中，我们评估了全长病毒基因组，从每四只猫感染PLV，以确定是否发生病毒重组，在这个单一的来源感染。在四只受感染的猫中，有三只的感染率是可测量的。在其中两只动物中，逆转录酶中的一个位点处于正选择状态，并且在基因组的3′半中的单个基因之间存在显著的拓扑不一致。断裂点接近用于辅助基因表达的剪接位点。我们的数据表明，重组可以促进慢病毒在不利环境中的持久性，如新的宿主物种。
Domestic cats develop an asymptomatic, productive infection with a feline immunodeficiency virus (PLV) derived from a naturally infected cougar (P. concolor). We previously demonstrated that there are extensive G to A substitutions, characteristic of host cytidine deaminase editing, and positive selection on reverse transcriptase in the PLV genome during this cross-species infection. In this study, we evaluated full-length viral genomes from each of four cats infected with PLV to determine if viral recombination occurred during this single source infection. Recombination rates were measurable in three of the four infected cats. In two of these animals, a single site in reverse transcriptase was under positive selection and there was significant topological incongruence among individual genes in the 3′ half of the genomes. The break point was proximate to a splice site used for accessory gene expression. Our data indicate that recombination can facilitate lentivirus persistence in unfavorable environments such as a new host species.