Integrated polymerase chain reaction-based procedures for the detection and identification of species and subspecies of the Gram-positive bacterial genus Lactococcus
Integrated polymerase chain reaction-based procedures for the detection and identification of species and subspecies of the Gram-positive bacterial genus Lactococcus
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DOI：
10.1046/j.1365-2672.2002.01688.x
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发表时间：
2002-01-01
影响因子：
4
通讯作者：
Powell, IB
中科院分区：
文献类型：
--
作者：
Pu, ZY;Dobos, M;Powell, IB
目的：对5种革兰氏阳性菌乳酸乳球菌（Lactococcus lactis，L。garvieae，L. [医]植物piscium和L.棉籽油乳）是目前公认的。这项工作的目的是开发一种简单的方法来鉴定这些物种，以及区分工业上重要的乳制品亚种L。lactis subsp. lactis和L. lactis subsp.方法和结果：方法设计的基础上，利用不同物种的16S核糖体RNA基因序列的差异，然后通过限制性内切酶切割的PCR产物的特异性聚合酶链反应（PCR）扩增。所开发的技术用于表征工业干酪发酵剂菌株的L。结论：所设计的PCR引物可同时使用，为未知菌株的筛选提供了一种简便的方法。菌株L. lactis显示异质性的16S核糖体RNA基因sequence.Significance和影响的研究：这项工作提供了一套完整的方法，用于区分和鉴定与农业，兽医，医疗和加工食品工业相关的乳球菌物种。
Aims: Five species of the Gram-positive bacterial genus Lactococcus (Lactococcus lactis , L. garvieae , L. plantarum , L. piscium and L. raffinolactis ) are currently recognized. The aim of this work was to develop a simple approach for the identification of these species, as well as to differentiate the industrially important dairy subspecies L. lactis subsp. lactis and L. lactis subsp. cremoris.Methods and Results: Methods were devised based on specific polymerase chain reaction (PCR) amplifications that exploit differences in the sequences of the 16S ribosomal RNA genes of each species, followed by restriction enzyme cleavage of the PCR products. The techniques developed were used to characterize industrial cheese starter strains of L. lactis and the results were compared with biochemical phenotype and DNA sequence data.Conclusions: The PCR primers designed can be used simultaneously, providing a simple scheme for screening unknown isolates. Strains of L. lactis show heterogeneity in the 16S ribosomal RNA gene sequence.Significance and Impact of the Study: This work provides an integrated set of methods for differentiation and identification of lactococcal species associated with agricultural, veterinary, medical and processed food industries.