Selenium protects sorghum leaves from oxidative damage under high temperature stress by enhancing antioxidant defense system
Selenium protects sorghum leaves from oxidative damage under high temperature stress by enhancing antioxidant defense system
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DOI：
10.1016/j.plaphy.2010.09.009
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发表时间：
2010-12-01
影响因子：
6.5
通讯作者：
Seppanen, M.
中科院分区：
文献类型：
--
作者：
Djanaguiraman, M.;Prasad, P. V. V.;Seppanen, M.
当植物在高温（HT）胁迫条件下生长时，通常会引发氧化胁迫。硒通常在植物中充当抗氧化剂；然而，其在 HT 诱导的氧化应激下的作用尚不明确。我们假设施硒可以部分缓解HT诱导的氧化应激以及HT对高粱[Sorghum bicolor (L.) Moench]生理、生长和产量的负面影响。本研究的目的是研究硒对受控环境中高温胁迫下生长的高粱植物的 (a) 叶片光合作用、膜稳定性和抗氧化酶活性以及 (b) 籽粒产量和产量构成的影响。从播种到播种后 63 天 (DAS)，植物在最佳温度（OT；白天最高/夜间最低 32/22 摄氏度）下生长。所有植物在 63 DAS 时叶面喷洒硒酸钠 (75 mg L-1)，并从 65 DAS 至成熟期间施加 HT 胁迫（40/30 摄氏度）。测量了生理、生化和产量性状的数据。高温胁迫降低叶绿素含量、叶绿素a荧光、光合速率和抗氧化酶活性，增加氧化剂产生和膜损伤。与 OT 相比，HT 胁迫下抗氧化防御能力下降导致谷物产量降低。硒的施用通过增强抗氧化防御来减少膜损伤，从而提高谷物产量。硒对抗氧化酶活性的增加和活性氧（ROS）含量的降低在HT中比在OT中更大。本研究表明，硒可以通过增强抗氧化防御系统在 HT 应激期间发挥保护作用。 (C) 2010 Elsevier Masson SAS。版权所有。
Oxidative stress is commonly induced when plants are grown under high temperature (HT) stress conditions. Selenium often acts as an antioxidant in plants; however, its role under HT-induced oxidative stress is not definite. We hypothesize that selenium application can partly alleviate HT-induced oxidative stress and negative impacts of HT on physiology, growth and yield of grain sorghum [Sorghum bicolor (L.) Moench]. Objectives of this study were to investigate the effects of selenium on (a) leaf photosynthesis, membrane stability and antioxidant enzymes activity and (b) grain yield and yield components of grain sorghum plants grown under HT stress in controlled environments. Plants were grown under optimal temperature (OT; 32/22 degrees C daytime maximum/nighttime minimum) from sowing to 63 days after sowing (DAS). All plants were foliar sprayed with sodium selenate (75 mg L-1) at 63 DAS, and HT stress (40/30 degrees C) was imposed from 65 DAS through maturity. Data on physiological, biochemical and yield traits were measured. High temperature stress decreased chlorophyll content, chlorophyll a fluorescence, photosynthetic rate and antioxidant enzyme activities and increased oxidant production and membrane damage. Decreased antioxidant defense under HT stress resulted in lower grain yield compared with OT. Application of selenium decreased membrane damage by enhancing antioxidant defense resulting in higher grain yield. The increase in antioxidant enzyme activities and decrease in reactive oxygen species (ROS) content by selenium was greater in HT than in OT. The present study suggests that selenium can play a protective role during HT stress by enhancing the antioxidant defense system. (C) 2010 Elsevier Masson SAS. All rights reserved.