Induction of heme oxygenase 1 in the retina by intense visible light: suppression by the antioxidant dimethylthiourea.
Induction of heme oxygenase 1 in the retina by intense visible light: suppression by the antioxidant dimethylthiourea.
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DOI：
10.1097/00006982-199616040-00030
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发表时间：
1995-02
影响因子：
11.1
通讯作者：
R. K. Kutty;G. Kutty;B. Wiggert;G. Chader;R. Darrow;D. Organisciak
中科院分区：
文献类型：
--
作者：
R. K. Kutty;G. Kutty;B. Wiggert;G. Chader;R. Darrow;D. Organisciak
研究了强可见光(光损伤)对氧化应激诱导的大鼠视网膜血红素氧合酶1(HO-1)蛋白表达的影响。一种灵敏的逆转录-聚合酶链式反应分析表明，在正常视网膜中，HO-1和非诱导型HO-2的mRNA都有表达。然而，Northern blotting分析表明，正常情况下几乎检测不到HO-1mRNA。在强烈可见光照射后，视网膜HO-1mRNA水平明显高于未暴露的对照组，在暴露12小时和24小时时分别增加了52倍和98倍。强光照射还导致HO-1蛋白表达增加。相反，未观察到HO-2mRNA或蛋白的明显变化。在以前在黑暗中饲养的大鼠中，HO-1信息的增加比在弱周期光环境中饲养的大鼠更明显。光照后在黑暗中恢复24小时后，HO-1mRNA的表达水平明显低于光刺激诱导的高水平。最重要的是，在光照前用1，3-二甲基硫脲(一种合成抗氧化剂)处理动物，有效地阻止了HO-1mRNA的增加。因此，HO-1是评估视网膜光诱导损伤的一个敏感标记物。由于HO-1的表达增加被认为是细胞对氧化损伤的防御，它的表达可能在保护视网膜免受光损伤方面发挥重要作用。
The effect of intense visible light (light damage) on the expression of heme oxygenase 1 (HO-1), a protein induced by oxidative stress, was investigated in the rat retina. A sensitive reverse transcription-PCR assay demonstrated the expression of mRNA for HO-1 as well as HO-2, the noninducible HO form, in the normal retina. As analyzed by Northern blotting, however, HO-1 mRNA was barely detectable under normal circumstances. After exposure to intense visible light, retinas had markedly higher HO-1 mRNA levels than unexposed controls, with increases up to 52- and 98-fold at 12 and 24 hr of exposure, respectively. Intense light exposure also resulted in an increase in HO-1 protein. In contrast, no appreciable change in HO-2 mRNA or protein was observed. The increase in HO-1 message was more pronounced in rats previously reared in the dark than in those reared in a weak cyclic-light environment. A marked decrease from the high level of HO-1 mRNA induced by light insult was observed when the animals were allowed to recover in the dark for 24 hr after light exposure. Most important, treatment of animals with 1,3-dimethylthiourea, a synthetic antioxidant, prior to light exposure effectively blocked the increase in HO-1 mRNA. Thus, HO-1 is a sensitive marker for assessing light-induced insult in the retina. Since increased expression of HO-1 is thought to be a cellular defense against oxidative damage, its expression may play an important role in protecting the retina against light damage.