EGCG, a green tea polyphenol, inhibits human coronavirus replication in vitro.
EGCG, a green tea polyphenol, inhibits human coronavirus replication in vitro.
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DOI：
10.1016/j.bbrc.2021.02.016
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发表时间：
2021-04-02
影响因子：
3.1
通讯作者：
Park J
中科院分区：
文献类型：
--
作者：
Jang M;Park R;Park YI;Cha YE;Yamamoto A;Lee JI;Park J
COVID-19大流行导致许多国家的死亡人数创历史新高。虽然现在已经有了SARS-CoV-2的疫苗，但还没有有效的抗病毒药物来治疗冠状病毒疾病。近年来，有报道称，一种绿色茶多酚--EGCG可抑制SARS-CoV-2 3CL-蛋白酶，但其对冠状病毒复制的影响尚不清楚。在这份报告中，人类冠状病毒HCoV-OC 43（β冠状病毒）和HCoV-229 E（α冠状病毒）被用来检查EGCG对冠状病毒的影响。EGCG处理降低了HCoV-0 C43和HCoV-229 E的3CL-蛋白酶活性。此外，EGCG处理降低了HCoV-OC 43诱导的细胞毒性。最后，我们发现EGCG处理降低了感染细胞培养基中冠状病毒RNA和蛋白质的水平。这些结果表明，EGCG抑制冠状病毒复制。
COVID-19 pandemic results in record high deaths in many countries. Although a vaccine for SARS-CoV-2 is now available, effective antiviral drugs to treat coronavirus diseases are not available yet. Recently, EGCG, a green tea polyphenol, was reported to inhibit SARS-CoV-2 3CL-protease, however the effect of EGCG on coronavirus replication is unknown. In this report, human coronavirus HCoV-OC43 (beta coronavirus) and HCoV-229E (alpha coronavirus) were used to examine the effect of EGCG on coronavirus. EGCG treatment decreases 3CL-protease activity of HCoV-OC43 and HCoV-229E. Moreover, EGCG treatment decreased HCoV-OC43-induced cytotoxicity. Finally, we found that EGCG treatment decreased the levels of coronavirus RNA and protein in infected cell media. These results indicate that EGCG inhibits coronavirus replication.