THE INTERACTION BETWEEN THE IRON-RESPONSIVE ELEMENT-BINDING PROTEIN AND ITS COGNATE RNA IS HIGHLY DEPENDENT UPON BOTH RNA SEQUENCE AND STRUCTURE
THE INTERACTION BETWEEN THE IRON-RESPONSIVE ELEMENT-BINDING PROTEIN AND ITS COGNATE RNA IS HIGHLY DEPENDENT UPON BOTH RNA SEQUENCE AND STRUCTURE
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DOI：
10.1093/nar/21.19.4627
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发表时间：
1993-09-25
影响因子：
14.9
通讯作者：
HARFORD, JB
中科院分区：
文献类型：
--
作者：
JAFFREY, SR;HAILE, DJ;HARFORD, JB
为了评估 RNA 序列/结构对 RNA 与铁反应元件结合蛋白 (IRE-BP) 相互作用的影响，测试了 28 种改变的 RNA，作为与铁蛋白 H 链 IRE 相对应的 RNA 的竞争者。预测的 IRE 发夹环和 IRE 茎中特有的不配对胞嘧啶残基的所有变化均显着降低了 RNA 对 IRE-BP 的表观亲和力。类似地，通过增加或减少分隔它们的碱基对的数量来改变环和茎的未配对胞嘧啶的间距和/或方向，显着降低了作为竞争者的功效。据推测，IRE-BP与其同源RNA形成多重接触，并且这些接触协同作用，为这种相互作用的高亲和力提供了基础。
To assess the influence of RNA sequence/structure on the interaction RNAs with the iron-responsive element binding protein (IRE-BP), twenty eight altered RNAs were tested as competitors for an RNA corresponding to the ferritin H chain IRE. All changes in the loop of the predicted IRE hairpin and in the unpaired cytosine residue characteristically found in IRE stems significantly decreased the apparent affinity of the RNA for the IRE-BP. Similarly, alteration in the spacing and/or orientation of the loop and the unpaired cytosine of the stem by either increasing or decreasing the number of base pairs separating them significantly reduced efficacy as a competitor. It is inferred that the IRE-BP forms multiple contacts with its cognate RNA, and that these contacts, acting in concert, provide the basis for the high affinity of this interaction.