Intracutaneous vaccination of rabbits with the cottontail rabbit papillomavirus (CRPV) L1 gene protects against virus challenge .
Intracutaneous vaccination of rabbits with the cottontail rabbit papillomavirus (CRPV) L1 gene protects against virus challenge .
复制标题
给兔子皮内接种棉尾兔乳头瘤病毒 (CRPV) L1 基因疫苗可以防止病毒攻击。
DOI：
10.1016/s0264-410x(96)00237-x
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发表时间：
1997
期刊：
影响因子：
5.5
通讯作者：
Brandsma,JL
中科院分区：
文献类型：
--
作者：
Sundaram,P;Tigelaar,RE;Brandsma,JL
构建了编码棉尾兔乳头瘤病毒(CRPV)主要衣壳蛋白L1的DNA疫苗。用一种特殊的递送装置(“基因枪”)将超螺旋质粒与金珠结合在一起。L1 DNA免疫的兔对CRPV病毒样颗粒产生细胞增殖反应，并产生对CRPV具有中和活性的高滴度抗体。在用CRPV进行实验性攻击后，所有L1DNA疫苗接种的兔与没有接种CRPV的对照兔相比，都能防止乳头状瘤的形成。这些结果表明，I.C.用L1乳头瘤病毒衣壳基因免疫兔子可以诱导抗体，以防止随后的乳头瘤病毒感染。
A DNA vaccine encoding the major capsid protein L1 of cottontail rabbit papillomavirus (CRPV) was constructed and administered intracutaneously (i.c.) to rabbits as supercoiled plasmids bound to gold beads using a specialized delivery device (“gene gun”). L1 DNA-vaccinated rabbits developed cellular proliferative responses to CRPV virus-like particles and developed high titered antibodies with neutralizing activity to CRPV. Following experimental challenge with CRPV, all of the L1 DNA-vaccinated rabbits, vs none of the controls, were protected from papilloma formation. These results demonstrate that i.c. vaccination of rabbits with the L1 papillomavirus capsid gene can induce antibodies that protect against subsequent papillomavirus infection.