A MOLECULAR LINKAGE MAP OF CULTIVATED OAT
A MOLECULAR LINKAGE MAP OF CULTIVATED OAT
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DOI：
10.1139/g95-048
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发表时间：
1995-04-01
期刊：
影响因子：
3.1
通讯作者：
VANDEYNZE, A
中科院分区：
文献类型：
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作者：
ODONOUGHUE, LS;KIANIAN, SF;VANDEYNZE, A
利用71个重组自交系构建了由561个位点组成的栽培燕麦分子连锁图谱。Kanota和A.青花菜奥格尔这些位点主要是限制性片段长度多态性检测燕麦cDNA克隆从叶片，胚乳，根组织，以及大麦叶片cDNA克隆。这些位点组成38个连锁群，连锁群大小为0.0 ~ 122.1cM，平均39 cM，每个连锁群由2-51个位点组成，平均14个位点。当前地图大小为1482 cM，根据未连锁基因座的数量，总大小估计为2932.0 cM。这表明该图谱覆盖了至少50%的栽培燕麦基因组。与一个A-基因组二倍体燕麦地图和连锁群之间表现出同源性彼此之间的比较表明，几个主要的染色体重排存在于栽培燕麦。该图谱为燕麦的分子标记辅助选择、数量性状位点分析和基因组结构研究提供了工具。
A molecular linkage map of cultivated oat composed of 561 loci has been developed using 71 recombinant inbred lines from a cross between Avena byzantina cv. Kanota and A. sativa cv. Ogle. The loci are mainly restriction fragment length polymorphisms detected by oat cDNA clones from leaf, endosperm, and root tissue, as well as by barley leaf cDNA clones. The loci form 38 linkage groups ranging in size from 0.0 to 122.1 cM (mean, 39 cM) and consist of 2-51 loci each (mean, 14), Twenty-nine loci remain unlinked. The current map size is 1482 cM and the total size, on the basis of the number of unlinked loci, is estimated to be 2932.0 cM. This indicates that this map covers at least 50% of the cultivated oat genome. Comparisons with an A-genome diploid oat map and between linkage groups exhibiting homoeology to each other indicate that several major chromosomal rearrangements exist in cultivated oat. This map provides a tool for marker-assisted selection, quantitative trait loci analyses, and studies of genome organization in oat.