Amino acid substitution in CAPRICE (CPC) protein affects its cell-to-cell movement in the root epidermis of Arabidopsis thaliana
Amino acid substitution in CAPRICE (CPC) protein affects its cell-to-cell movement in the root epidermis of Arabidopsis thaliana
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DOI：
10.1007/s13562-022-00810-4
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发表时间：
2022-10-08
影响因子：
1.9
通讯作者：
Tominaga，Rumi
中科院分区：
文献类型：
--
作者：
Idogawa，Arata;Qin，Dong;Tominaga，Rumi
CAPRICE（CPC）是一种单重复R3型MYB转录因子，通过从非根毛细胞转移到根毛细胞来调节根毛的形成。CPC同源蛋白TRY和CPC 1的增强子（ETC 1）不会在根表皮细胞之间移动。为了了解CPC的哪个区域参与细胞间的运动，通过替换ETC 1的特定氨基酸并将其转化到野生型拟南芥（Col-0）中来产生三种类型的嵌合构建体（嵌合体6、7和8）。研究了转基因植株的根表型、根毛和毛状体数量以及CPC蛋白的位置。我们前期的研究表明，表达CPC：：CPC：2× GFP和CPC：ETC 1：2× GFP的转基因植株比表达Col-0的转基因植株有更多的根毛。与CPC：：CPC：2× GFP和CPC：ETC 1：2×GFP相比，嵌合体6和7具有相似的根毛数量和GFP定位。然而，嵌合体8具有较低的根毛数，并且嵌合体8：GFP融合蛋白在一些细胞中不显示荧光。这些结果表明，嵌合体6和7中的两个氨基酸取代并不影响细胞间的运动能力，但是，将CPC的第24位半胱氨酸替换为ETC 1的第20位丝氨酸（嵌合体8）可能会影响CPC的根毛形成和细胞间的运动易位。我们的研究结果提供了深入了解CPC中的氨基酸可能有助于细胞间运动能力的差异。阐明CPC细胞间转移的机制将有助于更好地理解植物表皮细胞分化。
CAPRICE (CPC), a single-repeat R3-type MYB transcription factor, regulates root hair formation by transferring from non-root hair cells to root hair cells. The CPC homologue protein ENHANCER OF TRY AND CPC1 (ETC1) does not move between the cells in the root epidermis. To understand which region of the CPC is involved in cell-to-cell movement, three types of chimeric constructs (chimaeras 6, 7, and 8) were generated by replacing specific amino acids of ETC1, and transforming them into wild-typeArabidopsis thaliana(Col-0). The root phenotypes, root hair and trichome numbers, and location of the CPC protein in each transgenic plant were investigated. Our previous study indicated that transgenic plants expressingCPC::CPC:2×GFPandCPC:ETC1:2×GFPhad more root hair than Col-0. Compared toCPC::CPC:2×GFPandCPC:ETC1:2×GFP, the chimeras 6 and 7 had similar root hair numbers and GFP localizations. However, the chimera 8 had lower root hair numbers and chimera 8:GFP fusion proteins exhibited no fluorescence in some cells. These results suggest that the two amino acids substituted in chimeras 6 and 7 did not affect cell-to-cell movement ability; however, replacing the 24th cysteine of CPC with serine at the 20th position of ETC1 (chimera 8) could influence the root hair formation and cell-to-cell movement translocation of CPC. Our results provide insights into which amino acids in CPC may contribute to the difference in cell-to-cell movement ability. Elucidating the mechanism of cell-to-cell transfer of CPCs will lead to a better understanding of epidermal cell differentiation in plants.