FITNESS OF ARABIDOPSIS THALIANA MUTATION ACCUMULATION LINES WHOSE SPONTANEOUS MUTATIONS ARE KNOWN
FITNESS OF ARABIDOPSIS THALIANA MUTATION ACCUMULATION LINES WHOSE SPONTANEOUS MUTATIONS ARE KNOWN
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DOI：
10.1111/j.1558-5646.2012.01583.x
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发表时间：
2012-07-01
期刊：
影响因子：
3.3
通讯作者：
Fenster, Charles B.
中科院分区：
文献类型：
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作者：
Rutter, Matthew T.;Roles, Angela;Fenster, Charles B.
尽管突变对进化过程至关重要，但我们对特定的自发突变对有机体适应性的直接后果知之甚少。结合全基因组测序结果和重复的生存和繁殖田间分析，我们量化了在拟南芥中发现的自发突变对适应度的综合影响。我们证明，影响是有益的，有害的，或中性的，取决于环境背景。一些带有破坏已知基因座突变的品系，在适合度上与创始人或前突变基因型有很大不同。这些影响因环境而异，例如，在大多数情况下，转录因子基因重大缺失的品系表现出比创始人更低的适合度，但在一个环境中超过了创始人的适合度。基因与环境互作(G×E)对突变适合度效应的较大贡献暗示了新突变选择的空间和/或时间变异，并可能有助于维持现有的遗传变异。
Despite the fundamental importance of mutation to the evolutionary process, we have little knowledge of the direct consequences of specific spontaneous mutations to the fitness of the organism. Combining results of whole-genome sequencing with repeated field assays of survival and reproduction, we quantify the combined effects on fitness of spontaneous mutations identified in Arabidopsis thaliana. We demonstrate that the effects are beneficial, deleterious, or neutral depending on the environmental context. Some lines, bearing mutations disrupting known loci, differ strongly in fitness from the founder or premutation genotype. Those effects vary across environments, for example, a line with a major deletion spanning a transcription factor gene expressed lower fitness than the founder under most conditions but exceeded the founder's fitness in one environment. The large contribution of genotype by environment interaction (G x E) to mutation effects on fitness implies spatial and/or temporal variation in selection on new mutations and could contribute to the maintenance of standing genetic variation.