Non-lethal heat shock induces Hsp70 synthesis and promotes tolerance against heat, ammonia and metals in post-larvae of the white leg shrimp Penaeus vannamei (Boone, 1931)
Non-lethal heat shock induces Hsp70 synthesis and promotes tolerance against heat, ammonia and metals in post-larvae of the white leg shrimp Penaeus vannamei (Boone, 1931)
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DOI：
10.1016/j.aquaculture.2017.09.034
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发表时间：
2018-01-20
期刊：
影响因子：
4.5
通讯作者：
MacRae, Thomas H.
中科院分区：
文献类型：
--
作者：
Sung, Yeong Yik;Rahman, Nooridayu Abdul;MacRae, Thomas H.
暴露于高于环境的温度会损害正常的生理过程并对水生生物产生不利影响。然而，非致死性热休克（NLHS）保护水生生物免受非生物和生物胁迫，可能部分是由于热休克蛋白70（Hsp70），一个保守的分子伴侣的积累。Hsp70通过帮助错误折叠的蛋白质的恢复、促进新生蛋白质的折叠和促进蛋白质降解来保护细胞免受应激诱导的损伤。在这项研究中，用Hsp70特异性抗体对含有来自南美白对虾加热和非加热后幼虫（PL）尾部肌肉的蛋白提取物的蛋白质印迹进行免疫探测，结果表明NLHS增强了Hsp70在尾部的积聚。虾PL的组织。此外，暴露于NLHS保护凡纳滨对虾PL免受热，氨和重金属的影响。本文的研究结果表明，Hsp70的合成响应NLHS作出了贡献，保护凡纳滨对虾PL对几种不同的非生物胁迫。这些发现可能有助于制定保护对虾在水产养殖过程中免受环境胁迫的策略。
Exposure to temperatures above ambient compromises normal physiological processes and adversely affects aquatic organisms. Nonetheless, non-lethal heat shock (NLHS) protects aquatic organisms against abiotic and biotic stress, perhaps due in part to the accumulation of heat shock protein 70 (Hsp70), a well conserved molecular chaperone. Hsp70 protects cells against stress-induced damage by assisting in the recovery of misfolded proteins, facilitating the folding of nascent proteins and promoting protein degradation. In this study, the immunoprobing of western blots containing protein extracts from the caudal muscle of heated and non-heated postlarvae (PL) of the shrimp, Penaeus vannamei with an antibody specific to Hsp70 revealed that NLHS enhanced Hsp70 build-up in the caudal tissues of shrimp PL. Moreover, exposure to NLHS protected P. vannamei PL against heat, ammonia and heavy metals. The findings in this paper indicate that Hsp70 synthesized in response to NLHS contributed to the protection of P. vannamei PL against several different abiotic stressors. Such findings are potentially useful in the formulation of strategies to protect Penaeid shrimps against environmental stresses during aquaculture.