FLA, which encodes a homolog of UBP, is required for chlorophyll accumulation and development of lemma and palea in rice
FLA, which encodes a homolog of UBP, is required for chlorophyll accumulation and development of lemma and palea in rice
复制标题
FLA 编码 UBP 的同源物，是水稻叶绿素积累和外稃和内稃发育所必需的
DOI：
10.1007/s00299-018-2368-4
复制
发表时间：
2019-01
影响因子：
6.2
通讯作者：
韩龙植
中科院分区：
文献类型：
--
作者：
马小定;张佳妮;韩冰;唐江红;崔迪;韩龙植
泛素特异性蛋白酶（UBPs）是一类分布广泛、高度保守的蛋白质，也是最重要的去泛素化酶家族成员。虽然酵母、哺乳动物和拟南芥中的UBPs的功能和进化已被广泛报道，但水稻中UBPs的功能和进化关系尚不清楚。本研究对水稻花叶色异常突变体（弗拉）进行了鉴定，该突变体表现出花器官和花粉发育异常以及叶片白化等多种发育缺陷。我们用定位克隆的方法分离了弗拉，发现它编码一种泛素特异性蛋白酶的同源物。弗拉是一个广泛表达的基因，在花器官中表达量最高。亚细胞定位分析表明弗拉是一种细胞膜蛋白。通过对水稻基因组数据库（http：//rice.plantbiolo gy.msu.edu）的检索，我们在水稻基因组中鉴定出35个UBP家族成员。系统发育分析表明，这些蛋白质可分为16个亚家族，其中弗拉属于G8亚家族。体外活性测定表明，弗拉不具有去泛素化活性。我们的数据表明，弗拉在水稻花器官和叶绿体的发育中起重要作用，但这种作用可能不涉及去泛素化活性，因为弗拉不具有活性位点和去泛素化活性。
Ubiquitin-specific proteases (UBPs) are widely distributed and highly conserved proteins and are also members.of the most important family of deubiquitination enzymes. Although the functions and phylogenies of UBPs from yeast,.mammals and Arabidopsis have been widely reported, the functions and evolutionary relationships of UBPs in rice remain.unclear. In this study, we characterized the rice flower and leaf color aberrant mutant (fla), which exhibited a variety of.developmental defects, including abnormal floral organs and pollen development, and leaf bleaching. We isolated FLA by.positional cloning and found that it encodes a homolog of ubiquitin-specific protease. FLA is a ubiquitously expressed gene.with the highest expression in floral organs. Subcellular localization analysis indicated that FLA is a cell membrane protein..Through searches of the rice genome database (http://rice.plant biolo gy.msu.edu), we identified 35 UBP family members in.the rice genome. These proteins were grouped into 16 subfamilies based on phylogenetic analysis, and FLA was found to.belong to the G8 subfamily. In vitro activity assays revealed that FLA does not have deubiquitination activity. Our data suggest.that FLA plays an important role in the development of floral organs and chloroplast in rice, but that this role probably.does not involve deubiquitination activity, because FLA does not have an active site and deubiquitination activity.
登录
查看更多内容
影响因子：
4.5
作者：
Tank EM;True HL
通讯作者：
True HL
影响因子：
3.9
作者：
C. Rao-Naik;J. Chandler;B. McArdle;J. Callis
通讯作者：
C. Rao-Naik;J. Chandler;B. McArdle;J. Callis
影响因子：
3.5
作者：
Burnett, B;Li, FS;Pittman, RN
通讯作者：
Pittman, RN
影响因子：
7.7
作者：
Balakirev, MY;Tcherniuk, SO;Chroboczek, J
通讯作者：
Chroboczek, J
影响因子：
7.2
作者：
Doelling, JH;Yan, N;Vierstra, RD
通讯作者：
Vierstra, RD