HJURP is a CENP-A chromatin assembly factor sufficient to form a functional de novo kinetochore.
HJURP is a CENP-A chromatin assembly factor sufficient to form a functional de novo kinetochore.
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DOI：
10.1083/jcb.201012017
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发表时间：
2011-07-25
期刊：
影响因子：
--
通讯作者：
Foltz DR
中科院分区：
文献类型：
--
作者：
Barnhart MC;Kuich PH;Stellfox ME;Ward JA;Bassett EA;Black BE;Foltz DR
组蛋白伴侣HJURP是一种染色质组装因子，其将CENP-A核小体募集到着丝粒染色质。高等真核生物的着丝粒由着丝粒特异性CENP-A核小体表观遗传标记。新的CENP-A招募需要CENP-A组蛋白伴侣HJURP。在本文中，我们表明，一个LacI（紫胶阻遏物）融合的HJURP驱动的稳定招聘CENP-A的一个LacO（紫胶操纵子）阵列在noncentromeric位点。LacO阵列上的异位靶向CENP-A染色质足以指导功能性着丝粒的组装，如组成型着丝粒相关网络蛋白、微管结合蛋白NDC 80的募集以及稳定的着丝粒-微管附着的形成所示。HJURP的氨基末端片段能够在体外组装CENP-A核小体，这表明HJURP是一种染色质组装因子。此外，HJURP向内源性着丝粒的募集需要Mis 18复合体。总之，这些数据表明，Mis 18复合物在CENP-A沉积中的作用是招募HJURP，并且HJURP的CENP-A核小体组装活性负责着丝粒染色质组装以维持表观遗传标记。
The histone chaperone HJURP is a chromatin assembly factor that recruits CENP-A nucleosomes to centromeric chromatin. Centromeres of higher eukaryotes are epigenetically marked by the centromere-specific CENP-A nucleosome. New CENP-A recruitment requires the CENP-A histone chaperone HJURP. In this paper, we show that a LacI (Lac repressor) fusion of HJURP drove the stable recruitment of CENP-A to a LacO (Lac operon) array at a noncentromeric locus. Ectopically targeted CENP-A chromatin at the LacO array was sufficient to direct the assembly of a functional centromere as indicated by the recruitment of the constitutive centromere-associated network proteins, the microtubule-binding protein NDC80, and the formation of stable kinetochore–microtubule attachments. An amino-terminal fragment of HJURP was able to assemble CENP-A nucleosomes in vitro, demonstrating that HJURP is a chromatin assembly factor. Furthermore, HJURP recruitment to endogenous centromeres required the Mis18 complex. Together, these data suggest that the role of the Mis18 complex in CENP-A deposition is to recruit HJURP and that the CENP-A nucleosome assembly activity of HJURP is responsible for centromeric chromatin assembly to maintain the epigenetic mark.
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影响因子：
16
作者：
Camahort, Raymond;Shivaraju, Manjunatha;Mattingly, Mark;Li, Bing;Nakanishi, Shima;Zhu, Dongxiao;Shilatifard, Ali;Workman, Jerry L.;Gerton, Jennifer L.
通讯作者：
Gerton, Jennifer L.
影响因子：
21.3
作者：
Carroll, Christopher W.;Silva, Mariana C. C.;Godek, Kristina M.;Jansen, Lars E. T.;Straight, Aaron F.
通讯作者：
Straight, Aaron F.
影响因子：
10.5
作者：
Drane, Pascal;Ouararhni, Khalid;Hamiche, Ali
通讯作者：
Hamiche, Ali
影响因子：
64.5
作者：
Goldberg AD;Banaszynski LA;Noh KM;Lewis PW;Elsaesser SJ;Stadler S;Dewell S;Law M;Guo X;Li X;Wen D;Chapgier A;DeKelver RC;Miller JC;Lee YL;Boydston EA;Holmes MC;Gregory PD;Greally JM;Rafii S;Yang C;Scambler PJ;Garrick D;Gibbons RJ;Higgs DR;Cristea IM;Urnov FD;Zheng D;Allis CD
通讯作者：
Allis CD
DOI：
10.1083/jcb.201001013
发表时间：
2010-06-28
期刊：
The Journal of cell biology
影响因子：
--
作者：
Carroll CW;Milks KJ;Straight AF
通讯作者：
Straight AF