Corneal epithelium-specific mouse keratin K12 promoter
Corneal epithelium-specific mouse keratin K12 promoter
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DOI：
10.1006/exer.1998.0593
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发表时间：
1999-03-01
影响因子：
3.4
通讯作者：
Wilson, SE
中科院分区：
文献类型：
--
作者：
Liu, JJ;Kao, WWY;Wilson, SE
角蛋白是上皮细胞表达的结构蛋白。已经鉴定出大约30种不同的角蛋白，每种都在不同的上皮细胞中具有特定的表达模式。一些角蛋白基因的组织特异性启动子已被用于指导动物转基因的表达。角蛋白K12和K3在分化和分层的角膜上皮中表达，尽管它们的相对表达在物种之间有所不同。我们以小鼠K12角蛋白基因为目标，开发了一种组织特异性启动子，可用于研究角膜上皮中表达的相关基因的功能。角蛋白K12启动子的3个5'截断片段（1.03,0.71和0.25 Kb）在人角膜上皮细胞系中显示出比其他细胞系更高的功能和组织特异性启动子活性。0.25 Kb的K12启动子片段在培养的兔角膜上皮细胞中也有活性。因此，由小鼠K12启动子片段引导的角膜上皮细胞中表达的增加跨越了物种系。参与控制眼睛发育的配对箱形同源基因6 （PAX-6）刺激了角蛋白K12启动子的活性。(C) 1999学术出版社。
Keratins are structural proteins expressed by epithelial cells. Approximately 30 different keratin proteins have been identified, each with a specific expression pattern in different epithelial cells. The tissue-specific promoter of several keratin genes have been used to direct the expression of transgenes in animals. Keratin K12 and K3 are expressed in differentiated and stratified corneal epithelium, although the relative expression of each appears to vary between species. We targeted the mouse K12 keratin gene in order to develop a tissue-specific promoter that could be utilized to study the functions of genes of interest expressed in the corneal epithelium. Three 5' truncated fragments of the keratin K12 promoter (1.03, 0.71 and 0.25 Kb) showed higher functional and tissue-specific promoter activity in a human corneal epithelial cell line than other cell lines. The 0.25 Kb K12 promoter fragment was also active in cultured rabbit corneal epithelial cells. Thus, increased expression in corneal epithelial cells directed by fragments of the mouse K12 promoter extended across species lines. The paired box homeotic gene 6 (PAX-6), which is involved in controlling eye development, stimulated the activity of keratin K12 promoter. (C) 1999 Academic Press.