Evaluation of CD4+CD25+FOXP3+ regulatory T cells function in patients with common variable immunodeficiency
Evaluation of CD4+CD25+FOXP3+ regulatory T cells function in patients with common variable immunodeficiency
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DOI：
10.1016/j.cellimm.2013.03.003
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发表时间：
2013-02-01
影响因子：
4.3
通讯作者：
Aghamohammadi, Asghar
中科院分区：
文献类型：
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作者：
Arandi, Nargess;Mirshafiey, Abbas;Aghamohammadi, Asghar
常见变异型免疫缺陷（CVID）是一种主要的抗体疾病，其中调节性T细胞（TCLs）的异常可导致自身免疫和慢性炎症。为了评估Tegs频率和功能，招募了13名CVID患者和10名年龄和性别匹配的健康志愿者。采用流式细胞术计算T3细胞百分比。为了评估Treg功能，分离Tcl 3并使用Tcl 3抑制测定来确定其抑制功能。还测量了由TGFs产生的免疫调节细胞因子IL-10和TGF-β的水平。结果显示CVID患者TMPs频率（P < 0.001）和TMPs抑制功能（P < 0.001）均受损。CVID患者和对照组之间的TGF-β水平没有差异（p = 0.09）;而CVID患者中IL-10的量显著降低（P = 0.007）。我们的研究结果表明，干扰THBE的频率和他们的功能特点可能占异常免疫反应观察CVID患者。(C)2013 Elsevier Inc. All rights reserved.
Common variable immunodeficiency (CVID) is one of the predominant antibody disorders where abnormalities in regulatory T cells (Tregs) may result in autoimmunity and chronic inflammation. To evaluate Tegs frequency and function, 13 CVID patients and 10 age- and sex-matched healthy volunteer were enrolled. The percentages of Tregs were calculated using flow cytomety method. For assessment of Treg function, Tregs were isolated and their suppressive functions were determined using Tregs suppression assay. The levels of immunoregulatory cytokines IL-10 and TGF-beta produced by Tregs were also measured. Our results revealed that Tregs frequency (P < 0.001) and their suppressive functions (P < 0.001) were impaired in CVID patients. The level of TGF-beta did not differ between CVID patients and controls (p = 0.09); while the amount of IL-10 was remarkably decreased in CVID patients (P = 0.007). Our findings suggest that disturbed Tregs frequency and their functional characteristics might account for aberrant immune responses observed in CVID patients. (C) 2013 Elsevier Inc. All rights reserved.