Simultaneous biological removal of nitrogen and phosphorus in a vertical bioreactor
Simultaneous biological removal of nitrogen and phosphorus in a vertical bioreactor
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DOI：
10.1016/j.jece.2015.10.035
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发表时间：
2016-03
影响因子：
7.7
通讯作者：
M. Reza;M. Cuenca
中科院分区：
文献类型：
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作者：
M. Reza;M. Cuenca
营养物质污染已成为全球性的环境威胁。很大一部分营养物污染是由点源造成的，比如未经处理的生活和工业废水的排放。因此，迫切需要开发可靠、紧凑、高效的营养去除技术。在废水处理行业，大多数生物反应器的占地面积都很大，呈平面配置。此外，现有的营养去除工艺是基于众所周知的亚硝化细菌属和参与硝化/反硝化作用的亚硝化细菌和负责生物除磷的假丝酵母(Candidatus Acumulibater Php)。本工作在两个方面是前所未有的：(1)在多级立式管式生物反应器中进行了废水的生物脱氮除磷；(2)在该立式生物反应器中，两种丰富的微生物物种负责同时进行硝化-反硝化-BPR。丰富的微生物种群包括一种未鉴定的腐生菌属细菌和隶属于动物胶菌属的细菌。本研究所用人工合成废水的组成为：总磷(TP)32.6±100.7 mg/L，总氮(TN)272±77.5 mg/L，其中氨氮(NH3-N)45g±91.8 mg/L。该生物反应器在恒流、恒温、恒pH条件下连续运行350d以上，恒流温度为240℃(L/天)，恒温为22℃~24℃，pH为7~7.5。结果表明，同步硝化-反硝化-BPR是微生物种群的主导过程，但尚未完全分类。出水总磷和总氮浓度分别为2.7g±0.04g/L和4.3g±1.2g/g L。出水中NH_3-N、NO_2、−和NO_3-N的浓度分别为0.7g±0.01g/L、0.8g±0.05g/g和0.3g±0.1g/g。同步硝化-反硝化-BPR工艺对TN和TP的去除效率均在90%以上。本文介绍的成功成果已导致在安大略省皮克林建设了一座20,000吨L/天的示范工厂。
Nutrients pollution has become a global environmental threat. A large fraction of nutrient pollution is caused by point sources like the discharges of untreated domestic and industrial wastewater. As a result, there is a great demand to develop reliable, compact and efficient nutrient removal technologies. In the wastewater industry, most of the bioreactors have a large foot print with a plane configuration. Furthermore, the existing nutrient removal processes are based on well-known microbial species of genusNitrosomonasandNitrobacterinvolved in nitrification/denitrification andCandidatus Accumulibater Phosphatisresponsible for biological phosphorous removal (BPR). The present work is unprecedented in two aspects: (1) The biological nitrogen and phosphorus removal from wastewater occurred in a multistage vertical, tubular bioreactor and (2) Two abundant microbial species were responsible for the simultaneous nitrification–denitrification–BPR in this vertical bioreactor. The abundant microbial populations included an unidentified bacteria ofSaprospiraceaefamily and bacteria affiliated with the genusZoogloea. The composition of the synthetic wastewater used in this study was: total phosphorous (TP) 32.6 ± 0.7 mg/L, total nitrogen (TN) 272 ± 7.5 in which 45 ± 1.8 mg/L was ammonia-nitrogen (NH3-N). The bioreactor was continuously operated for over 350 days at constant flowrate, temperature and pH of 240 (L/day), 22–24 (°C) and 7–7.5. The results showed that simultaneous nitrification–denitrification–BPR was the dominant process by an, as yet not fully classified, microbial species. The effluent TP and TN concentrations were 2.7 ± 0.4 and 4.3 ± 1.2 mg/L respectively. Concentrations of NH3-N, NO2−and NO3-N in the effluent were 0.7 ± 0.1, 0.8 ± 0.5 and 0.3 ± 0.1. The simultaneous nitrification–denitrification–BPR was highly effective delivering above 90% TN and TP removal efficiency. The successful results presented in this paper have led to the construction of a 20,000 L/day demonstration plant in the City of Pickering, Ontario.