CONTINUOUS CELL-LINES AND IMMUNE ASCITIC FLUID POOLS IN ARBOVIRUS DETECTION
CONTINUOUS CELL-LINES AND IMMUNE ASCITIC FLUID POOLS IN ARBOVIRUS DETECTION
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DOI：
10.1016/s0923-2516(06)80135-4
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发表时间：
1992-11-01
期刊：
影响因子：
--
通讯作者：
ADAM, F
中科院分区：
文献类型：
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作者：
DIGOUTTE, JP;CALVOWILSON, MA;ADAM, F
我们采用世界卫生组织合作中心推荐的虫媒病毒系统研究和分离的连续细胞系中的两种细胞系统，MOS 61（Aedes pseudoscutellaris cells）和Vero细胞，用含有10种参考病毒的超免疫腹水的7种混合物进行细胞培养中的病毒检测。该技术能够检测非洲蚊子传播的80种虫媒病毒中的70种，并通过使用间接免疫荧光技术中使用的单特异性或单克隆免疫腹水（登革热1-2-3-4和黄热病病毒）非常容易地检测虫媒病毒相关性。
Successive experiments led us to use two cellular systems, MOS61 (Aedes pseudoscutellaris cells) and Vero cells, among the continuous cell lines recommended by the WHO Collaborating Center for systematic research and isolation of arboviruses.Virus detection in cell cultures is carried out with 7 mixtures containing 10 hyperimmune ascitic fluids made with the reference viruses. This technique enables the detection of 70 of the 80 arboviruses transmitted by mosquitoes in Africa and very easily detects arbovirus associations by using either monospecific or monoclonal immune ascitic fluids (dengue-1-2-3-4 and yellow fever viruses) used in the indirect immunofluorescence technique.