Passive smoking induces pediatric asthma by affecting the balance of Treg/Th17 cells
Passive smoking induces pediatric asthma by affecting the balance of Treg/Th17 cells
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DOI：
10.1038/s41390-019-0276-0
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发表时间：
2019-03-01
影响因子：
3.6
通讯作者：
Liu, Qingbin
中科院分区：
文献类型：
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作者：
Jing, Wei;Wang, Wei;Liu, Qingbin
背景：本研究旨在探讨被动吸烟对儿童哮喘严重程度的影响及其分子机制。方法：将378例儿童哮喘患者按哮喘严重程度分为4组。使用单变量和多变量回归分析来分析与儿童哮喘严重程度相关的可能因素。环境烟草烟雾(ETS)暴露通过尿可替宁浓度进行测量。用变应原诊断试剂盒和酶联免疫吸附试验(ELISA)试剂盒检测血清免疫球蛋白E(IgE)和细胞因子水平。用流式细胞仪检测外周血单个核细胞(PMBC)中T细胞调节性T细胞(Treg)和辅助性T细胞17型(Th17)的百分比。结果：ETS和血清IgE水平、被动吸烟时间和吸烟量与哮喘严重程度密切相关。被动吸烟显著降低与Treg细胞相关的FoxP3(叉头/翼螺旋转录因子)和肿瘤生长因子-β的水平，升高与Th17细胞相关的IL-17A和IL-23的水平。同时，被动吸烟可显著降低Treg/Th17细胞的比例(P<；0.05)。结论：被动吸烟通过影响Treg/Th17细胞的平衡，与儿童哮喘的严重程度密切相关。
BACKGROUND: We aimed to explore the effects of passive smoking on the severity of pediatric asthma and associated molecular mechanisms.METHODS: A total of 378 children with asthma were assigned into four groups according to asthma severity (from grades I to IV). Univariate and multivariate regression analyses were used to analyze possible factors associated with asthma severity in children. Environmental tobacco smoke (ETS) exposure was measured via cotinine concentration in urine. Serum levels of immunoglobulin E (IgE) and cytokines were measured using allergen diagnostic and ELISA (enzyme-linked immunosorbent assay) kits. The percentage of T-regulatory (Treg) and T-helper type 17 (Th17) cells in peripheral blood mononuclear cells (PMBCs) were measured by flow cytometry. Treg- and Th17-associated transcription factors from PMBCs were measured by using ELISA kits.RESULTS: The levels of ETS and serum IgE, and the duration and amounts of passive smoking were closely associated with asthma severity. Passive smoking significantly reduced the levels of FoxP3 (Forkhead/winged helix transcription factor) and tumor growth factor-beta, which were associated with Treg cells, and increased the levels of interleukin-17A and interleukin-23, which were associated with Th17 cells. Meanwhile, passive smoking significantly reduced the ratio of Treg/Th17 cells (P < 0.05).CONCLUSIONS: Passive smoking was closely associated with the severity of childhood asthma by affecting the balance of Treg/Th17 cells.