Stimulation of the catalytic cycle of the Ca2+ pump of porcine plasma-membranes by negatively charged phospholipids.
Stimulation of the catalytic cycle of the Ca2+ pump of porcine plasma-membranes by negatively charged phospholipids.
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带负电的磷脂刺激猪质膜 Ca2 泵的催化循环。
DOI：
10.1016/0005-2736(92)90169-m
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Rik Casteels
中科院分区：
文献类型：
--
作者：
Jan Lehotsky;L. Raeymaekers;L. Missiaen;F. Wuytack;H. Smedt;Rik Casteels
质膜的（Ca 2 ++ Mg 2+）-ATP酶被带负电荷的磷脂激活。通过研究带负电荷的磷脂对稳态磷酸化中间体水平和对-硝基苯磷酸酶活性的影响，研究了这种激活的机制。不同的酸性磷脂对这两个参数的影响不同。磷蛋白中间体的水平不受磷脂酰酶（总磷脂的20%），但它增加了60%的磷脂酰肌醇4-磷酸。磷脂酰丝氨酸增加p-硝基苯磷酸酶活性，而磷脂酰肌醇4-磷酸没有显着影响。有人建议，磷脂酰肌醇4-磷酸主要影响一个反应步骤，导致加速形成的磷酸化中间体，而磷脂酰丝氨酸的行动将影响两个反应步骤，一个上游和下游的磷酸化中间体。
The (Ca2++Mg2+)-ATPase of the plasma membrane is activated by negatively charged phospholipids. The mechanism of this activation was investigated by studying the effect of negatively charged phospholipids on the steady state phosphointermediate level and on thep-nitrophenylphosphatase activity. Both the parameters were differentially affected by different acidic phospholipids. The level of phosphoprotein intermediate was not affected by phosphatidylse (20% of total phospholipid), but it was increased by 60% by phosphatidylinositol 4-phosphate. Phosphatidylserine increased thep-nitrophenylphosphatase activity, whereas phosphatidylinositol 4-phosphate had no significant effect. It is suggested that phosphatidylinositol 4-phosphate mainly affects a reaction step which leads to accelerated formation of the phosphointermediate, whereas the action of phosphatidylserine would affect two reaction steps, one upstream and one downstream of the phosphointermediate.
从红细胞膜纯化的钙依赖性三磷酸腺苷酶催化循环中的部分反应。
DOI：
--
发表时间：
1986
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Kosk-Kosicka,D;Scaillet,S;Inesi,G
通讯作者：
Inesi,G
通过自身结合或与钙调蛋白相互作用激活红细胞 Ca2 -ATP 酶。
DOI：
--
发表时间：
1988
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Kosk-Kosicka,D;Bzdega,T
通讯作者：
Bzdega,T