Organization of the nitrergic neuronal system in the primitive bony fishes Polypterus senegalus and Erpetoichthys calabaricus (Actinopterygii: Cladistia)
Organization of the nitrergic neuronal system in the primitive bony fishes Polypterus senegalus and Erpetoichthys calabaricus (Actinopterygii: Cladistia)
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DOI：
10.1002/cne.23922
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发表时间：
2016-06
影响因子：
2.5
通讯作者：
Jesús M. López;D. Lozano;R. Morona;Agustín González
中科院分区：
文献类型：
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作者：
Jesús M. López;D. Lozano;R. Morona;Agustín González
枝鳍鱼是一组基生辐鳍鱼类，是研究原始脑特征的良好模型，最有可能存在于祖先硬骨鱼中。对氮能神经元的分析（用一氧化氮合酶; NOS）有助于理解所有研究的脊椎动物脑组织的重要方面。我们通过NOS和NADPH-黄递酶（NADPH-d）组织化学的特异性抗体研究了两种支序纲物种的氮能系统，除了初级嗅觉和末端神经纤维，仅标记为NADPH-d，得到了相同的结果。双免疫组化进行同时检测NOS与酪氨酸羟化酶，胆碱乙酰转移酶，钙结合蛋白，钙视网膜蛋白，和5-羟色胺，建立准确的定位的氮能神经元和纤维，并评估这些神经活性物质之间可能的相互作用。两个物种中的分布模式仅显示标记细胞密度的细微差异。在苍白球和苍白球下区、室旁区、结节和乳头体后下丘脑区、后结节、丘脑前和丘脑区、视顶盖、枕叶、中脑被盖、脚间核、上级和中间网状核、前庭大细胞核、孤束核、正中大细胞核、脊髓和视网膜中的无长突细胞。脑神经感觉神经节中的大神经元也被标记。这些结果与其他脊椎动物的比较，使用neuromeric分析，揭示了一个保守的组织模式的nitrergic系统，从这个原始的鱼类种群，包括哺乳动物。J. Comp.神经元524：1770-1804，2016.© 2015威利期刊公司.
Cladistians are a group of basal actinopterygian fishes that constitute a good model for studying primitive brain features, most likely present in the ancestral bony fishes. The analysis of the nitrergic neurons (with the enzyme nitric oxide synthase; NOS) has helped in understanding important aspects of brain organization in all vertebrates studied. We investigated the nitrergic system of two cladistian species by means of specific antibodies against NOS and NADPH‐diaphorase (NADPH‐d) histochemistry, which, with the exception of the primary olfactory and terminal nerve fibers, labeled only for NADPH‐d, yielded identical results. Double immunohistochemistry was conducted for simultaneous detection of NOS with tyrosine hydroxylase, choline acetyltransferase, calbindin, calretinin, and serotonin, to establish accurately the localization of the nitrergic neurons and fibers and to assess possible interactions between these neuroactive substances. The pattern of distribution in both species showed only subtle differences in the density of labeled cells. Distinct groups of NOS‐immunoreactive cells were observed in pallial and subpallial areas, paraventricular region, tuberal and retromammillary hypothalamic areas, posterior tubercle, prethalamic and thalamic areas, optic tectum, torus semicircularis, mesencephalic tegmentum, interpeduncular nucleus, superior and middle reticular nuclei, magnocellular vestibular nucleus, solitary tract nucleus, nucleus medianus magnocellularis, the spinal cord and amacrine cells in the retina. Large neurons in cranial nerve sensory ganglia were also labeled. The comparison of these results with those from other vertebrates, using a neuromeric analysis, reveals a conserved pattern of organization of the nitrergic system from this primitive fish group to amniotes, including mammals. J. Comp. Neurol. 524:1770–1804, 2016. © 2015 Wiley Periodicals, Inc.