The response of normal human osteoblasts to anionic polysaccharide polyelectrolyte complexes
The response of normal human osteoblasts to anionic polysaccharide polyelectrolyte complexes
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DOI：
10.1016/j.biomaterials.2005.01.041
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发表时间：
2005-09-01
期刊：
影响因子：
14
通讯作者：
Tsuchiya, T
中科院分区：
文献类型：
--
作者：
Nagahata, M;Nakaoka, R;Tsuchiya, T
以壳聚糖为聚阳离子，合成了几种功能性阴离子多糖，制备了聚电解质复合物（PEC），并以正常人成骨细胞（NHOst）为模型，研究了PEC对细胞粘附、形态、增殖和分化的影响。孵育1周后，由具有羧基作为聚阴离子的多糖制成的PEC在其上显示出低的NHOst活力，尽管其上的NHOst显示出其分化水平的增强。另一方面，NHOst对PEC由硫酸化或磷酸化的多糖显示出类似的附着和形态上的胶原包被的盘。当1周后估计NHOst的数量时，PEC上的数量为胶原包被的培养皿上的数量的70%至130%，表明这些PEC对细胞增殖的影响很小。此外，由硫酸化多糖制成的PEC膜上的NHOst分化至与在胶原包被的培养皿上观察到的水平非常相似的水平，表明这些PEC膜保持NHOst增殖和分化的正常潜力。NHOst对PEC的间隙连接细胞间通讯的测量显示PEC不抑制通讯，表明PEC膜对细胞内稳态几乎没有影响。因此，由硫酸多糖制备的PEC可能是一种有用的材料，作为一种新的骨再生支架。(c)2005爱思唯尔有限公司保留所有权利。
Polyelectrolyte complexes (PEC) were prepared from chitosan as the polycation and several synthesized functional anion polysaccharides, and their effects on cell attachment, morphology, proliferation and differentiation were estimated using normal human osteoblasts (NHOst). After a 1-week incubation, PEC made from polysaccharides having carboxyl groups as polyanions showed low viability of NHOst on it although the NHOst on it showed an enhancement in their differentiation level. On the other hand, NHOst on PEC made from sulfated or phosphated polysaccharides showed similar attachment and morphology to those on the collagen-coated dish. When the number of NHOst was estimated after I week, the number on the PEC was ranged from 70% to 130% of those on the collagen-coated dish, indicating few effects of these PEC on cell proliferation. In addition, NHOst on PEC films made from sulfated polysaccharides differentiated to a level very similar to that observed on the collagen-coated dish, indicating that these PEC films maintain the normal potential of NHOst to both proliferate and differentiate. Measurement of gap junctional intercellular communication of NHOst on PEC revealed that PEC did not inhibit communication, suggesting that PEC films have few effects on cell homeostasis. Thus, PEC made from the sulfated polysaccharide may be a useful material as a new scaffold for bone regeneration. (c) 2005 Elsevier Ltd. All rights reserved.