A study of human tissue aminopeptidase components.
A study of human tissue aminopeptidase components.
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人体组织氨肽酶成分的研究。
DOI：
10.1016/0003-9861(65)90194-3
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发表时间：
1965
影响因子：
3.9
通讯作者：
J. Hardman
中科院分区：
文献类型：
--
作者：
F. J. Běhal;B. Asserson;F. Dawson;J. Hardman
研究了人肝、小肠和胰腺的氨肽酶活性。肝脏含有两种色谱可分辨类型的氨肽酶，一种是Mn++依赖性的，对二肽有活性，另一种不需要金属离子，对氨基酸β-萘酰胺（BNA）有活性。肠中含有一个Mn++依赖性氨肽酶活性二肽和Co++依赖性氨肽酶活性氨基酸BNA，同时从柱洗脱。胰腺也包含两种类型的氨肽酶活性;对氨基酸BNA的活性，钴++依赖，色谱解析成两个组件。在所有情况下，氨肽酶对二肽的活性对嘌呤霉素不敏感，而氨肽酶对氨基酸BNA的活性对嘌呤霉素相当敏感。
The aminopeptidase activity of human liver, small intestine, and pancreas was studied. Liver contained two chromatographically resolvable types of aminopeptidase, one Mn++-dependent and active on dipeptides and a second with no metal ion requirement and active on amino acid β-naphthylamides (BNA). Intestine contained a Mn++-dependent aminopeptidase active on dipeptides and a Co++-dependent aminopeptidase active on amino acid BNA which eluted simultaneously from a column. Pancreas also contained both types of aminopeptidase activity; the activity on amino acid BNA, Co++-dependent, was chromatographically resolved into two components. In all cases aminopeptidase activity on dipeptides was insensitive to puromycin while aminopeptidase activity on amino acid BNA was quite sensitive to puromycin.