NOVEL 68-KDA AUTOANTIGEN DETECTED BY RHEUMATOID-ARTHRITIS SPECIFIC ANTIBODIES
NOVEL 68-KDA AUTOANTIGEN DETECTED BY RHEUMATOID-ARTHRITIS SPECIFIC ANTIBODIES
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DOI：
10.1136/ard.54.5.355
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发表时间：
1995-05-01
影响因子：
27.4
通讯作者：
SCHWOCHAU, M
中科院分区：
文献类型：
--
作者：
BLASS, S;SPECKER, C;SCHWOCHAU, M
通过鉴定新的疾病特异性自身抗体，提高对类风湿性关节炎（RA）发病机制的认识。方法从滑膜中分离总蛋白制剂，进行免疫印迹。通过印迹法筛选RA患者血清的主要免疫反应。一个68 kDa的抗原目标的最主要的反应进行检测，并进一步characterized.Results-the占主导地位的免疫反应，在大多数的RA血清测试是与68 kDa的抗原。该抗原可能广泛表达。它的等电点为5.1，是O-糖基化的，位于内质网、细胞质或两者。68 kDa的自身抗原的抗体存在于64%的167例RA患者测试，也可以在血清阴性RA患者中检测到，但存在于98例其他风湿性疾病的患者中只有1%。他们不能被检测到在55健康controls. Conclusions，由于其高灵敏度（64%）和特异性（99%），抗68 kDa自身抗体不仅提供了另一个有价值的参数诊断，但也代表了抗体，可能参与的病理机制，导致RA。这一假设可以通过研究RA患者中是否存在68 kDa特异性T细胞来检验。
Objective-To improve the understanding of the pathogenesis of rheumatoid arthritis (RA) by identifying novel, disease specific autoantibodies.Methods-Total protein preparations from synovial membranes were separated electrophoretically and immunoblotted. Sera from RA patients were screened for predominant immunoreactions by blotting. A 68 kDa antigen target of the most predominant reaction was detected and further characterised.Results-The dominant immunoreaction in most of the RA sera tested was with a 68 kDa antigen. The antigen is probably ubiquitously expressed. It has an isoelectric point of 5.1, is O-glycosylated, and is located in the endoplasmic reticulum, the cytoplasm, or both. Antibodies to the 68 kDa autoantigen were present in 64% of 167 RA patients tested, and could also be detected in seronegative RA patients, but were present in only 1% of 98 patients with other rheumatic diseases. They could not be detected in 55 healthy controls.Conclusions-Because of its high sensitivity (64%) and specificity (99%), the anti-68 kDa autoantibody not only provides another valuable parameter for diagnosis, but also represents an antibody that may be involved in the pathological mechanisms leading to RA. This hypothesis can be tested by investigating if 68 kDa specific T cells are present in RA patients.