Pharmacokinetics of Five Different Types of Safflower Constituents in Rat Plasma after Oral Administration of Safflower Powder
Pharmacokinetics of Five Different Types of Safflower Constituents in Rat Plasma after Oral Administration of Safflower Powder
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DOI：
10.1080/10826076.2015.1093501
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发表时间：
2015-09
影响因子：
1.3
通讯作者：
Yerigui;Bulgantuya Bumandorj;Narantsetseg Byambaakhuu;Chao-Mei Ma
中科院分区：
文献类型：
--
作者：
Yerigui;Bulgantuya Bumandorj;Narantsetseg Byambaakhuu;Chao-Mei Ma
红花(Carthamus tinctorius L.)花瓣是重要的活血化瘀药。红花的多种成分的药代动力学特性还没有完全被了解。本研究建立并验证了动态多反应监测模式下的超高效液相色谱-三重四极杆质谱法同时测定大鼠血浆中查尔酮、聚乙炔、6-羟基山奈酚糖苷等5种不同特征类型红花成分的方法，同时检测到灌胃红花粉后大鼠血浆中的5种成分--羟基红花黄色素A、山梨苷C、6-羟基山奈酚-3，6-二葡萄糖苷、绿原酸和对香豆酸。对香豆酸吸收最快，Tmax为0.1hr。多乙炔成分二烯糖苷C的AUC0-t值最大(2177.796 ng·h/L)，其次是羟基红花黄色素A(1564.1 ng·h/L)。首次报道了野双烯皂苷C良好的药代动力学行为，这使得更多地关注它在红花体内药理作用中的作用变得重要。
The petals of Carthamus tinctorius L. (safflower) is an important traditional medicine for promoting blood circulation and removing blood stasis. The pharmacokinetic properties of multiple safflower constituents have not been fully understood. In the present research, an ultra-high-performance liquid chromatography-triple quadrupole mass method in dynamic multiple reaction monitoring mode was developed and validated to simultaneously quantify in rat plasma of five different characteristic types of safflower constituents, including chalcone, poly-acetylene, glycoside of 6-hydroxykaempferol, etc. All the five constituents, hydroxysafflor yellow A, bidenoside C, 6-hydroxykaempferol-3,6-diglucoside, chlorogenic acid, and p-coumaric acid were detected in rat plasma after oral administration of safflower powder. p-Coumaric acid was the most quickly absorbed one with Tmax of 0.1 hr. The poly-acetylene constituent, bidenoside C, displayed the largest AUC0-t value (2177.796 ng · h/L) followed by hydroxysafflor yellow A (1564.1 ng · h/L). The impressively favorable pharmacokinetic behavior of bidenoside C reported herein for the first time rendered the importance for paying more attention to its role in the in vivo pharmacologic effects of safflower.