Testosterone inhibits tumor necrosis factor-α-induced vascular cell adhesion molecule-1 expression in human aortic endothelial cells
Testosterone inhibits tumor necrosis factor-α-induced vascular cell adhesion molecule-1 expression in human aortic endothelial cells
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DOI：
10.1016/s0014-5793(02)03440-3
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发表时间：
2002-10-23
期刊：
影响因子：
3.5
通讯作者：
Uchida, K
中科院分区：
文献类型：
--
作者：
Hatakeyama, H;Nishizawa, M;Uchida, K
我们研究了睾酮(T)对人主动脉内皮细胞肿瘤坏死因子-a （tnf - α）诱导的血管细胞粘附分子-1 （VCAM-1）表达的影响。这些细胞与T的孵育导致表达的剂量依赖性减少，这种减少被选择性雄激素受体阻断剂完全消除。电泳迁移率转移实验表明，T可能通过抑制核易位来抑制tnf α诱导的转录核因子- kappab的激活，这对诱导VCAM-1的表达至关重要。我们的结果可能提示T对动脉粥样硬化的抑制作用，为考虑动脉粥样硬化的发病机制提供了新的见解。(C) 2002年欧洲生化学会联合会。Elsevier Science B.V.版权所有。
We investigated the effect of testosterone (T) on tumor necrosis factor-a (TNF-alpha)-induced expression of vascular cell adhesion molecule-1 (VCAM-1) in human aortic endothelial cells. Incubation of these cells with T resulted in a dose-dependent reduction in the expression, with this reduction completely abolished by a selective androgen receptor blocker. Electrophoretic mobility shift assay demonstrated that T inhibited TNF-alpha-induced activation of the transcriptional nuclear factor-kappaB, which is critical for the inducible expression of VCAM-1, probably through the suppression of the nuclear translocation. Our results may suggest an inhibitory effect of T on atherogenesis, providing a novel insight into the consideration of the pathogenesis of atherosclerosis. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.