Endogenous mast cell degranulation modulates cervical contractility in the guinea pig
Endogenous mast cell degranulation modulates cervical contractility in the guinea pig
复制标题
DOI：
10.1067/mob.2002.120488
复制
发表时间：
2002-03-01
影响因子：
9.8
通讯作者：
Garfield, RE
中科院分区：
文献类型：
--
作者：
Bytautiene, E;Vedernikov, YP;Garfield, RE
目的：探讨内源性肥大细胞脱颗粒对非妊娠和妊娠豚鼠离体颈肌条收缩性能的影响。研究设计：用非妊娠和妊娠(中期和足月)豚鼠的纵向颈条进行等长张力记录。比较了肥大细胞脱粒剂化合物48/80在不同抑制剂和受体拮抗剂存在或不存在的情况下的反应。结果：化合物48/80和组胺对3组动物颈条的收缩均有明显的促进作用。肥大细胞脱颗粒抑制剂显著降低对化合物48/80的反应，组胺-1受体拮抗剂降低所有3组对组胺的反应。组胺-1受体拮抗剂显著抑制未孕和中期妊娠豚鼠对化合物48/80的反应。组胺-2受体拮抗剂不改变化合物48/80的反应，也不改变组胺的反应。受体拮抗剂5-羟色胺-2显著抑制化合物48/80在中期妊娠和足月妊娠豚鼠组织中引起的宫颈收缩。脂氧合酶抑制剂对中期妊娠豚鼠有效。环氧合酶抑制剂5-羟色胺以及脂氧合酶和环氧合酶抑制剂的组合对宫颈收缩无影响。结论：肥大细胞脱颗粒释放组胺等介质，通过组胺-1受体刺激宫颈收缩。肥大细胞对宫颈的侵袭和收缩功能的调节可能在孕期宫颈功能调控中发挥重要的生理和/或病理作用。
OBJECTIVE: The purpose of this study was to investigate the effect of endogenous mast cell degranulation on the contractility of isolated cervical strips from nonpregnant and pregnant guinea pigs.STUDY DESIGN: Longitudinal cervical strips from nonpregnant and pregnant (mid and term) guinea pigs were used for isometric tension recording. Responses to the mast cell degranulating agent, compound 48/80, were compared in the absence or presence of different inhibitors and receptor antagonists. Concentration-response curves were obtained to histamine and 5-hydroxytryptamine in strips that were incubated with antagonists or solvent.RESULTS: Compound 48/80 and histamine significantly increased contractility of cervical strips in all 3 groups of animals. The inhibitor of mast cell degranulation significantly reduced responses to compound 48/80 and histamine-1 receptor antagonist reduced responses to histamine in all 3 groups. Histamine-1 receptor antagonist significantly inhibited responses to compound 48/80 in nonpregnant and mid pregnant guinea pigs. Histamine-2 receptor antagonist did not alter responses to compound 48/80 nor to histamine. The receptor antagonist 5-hydroxytryptamine-2 significantly inhibited cervical contractility that was induced by compound 48/80 in tissues from mid pregnant and term pregnant guinea pigs. Lipoxygenase inhibitor was effective in mid pregnant guinea pigs. Cyclooxygenase inhibitor, 5-hydroxytryptamine, and a combination of lipoxygenase and cyclooxygenase inhibitors had no effect on cervical contractility.CONCLUSION: The degranulation of mast cells releases histamine and other mediators that stimulate cervical contractility through histamine-1 receptors. Cervical infiltration and modulation of contractility by mast cells may play an important physiologic and/or pathologic role in the control of cervical function during pregnancy.