Goldfish ghrelin: molecular characterization of the complementary deoxyribonucleic acid, partial gene structure and evidence for its stimulatory role in food intake.
Goldfish ghrelin: molecular characterization of the complementary deoxyribonucleic acid, partial gene structure and evidence for its stimulatory role in food intake.
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DOI：
10.1210/en.2002-220644
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发表时间：
2002-10
期刊：
影响因子：
4.8
通讯作者：
S. Unniappan;Xin-wei Lin;L. Cervini;J. Rivier;H. Kaiya;K. Kangawa;R. Peter
中科院分区：
文献类型：
--
作者：
S. Unniappan;Xin-wei Lin;L. Cervini;J. Rivier;H. Kaiya;K. Kangawa;R. Peter
采用cDNA末端快速扩增（RACE）和逆转录(RT)-聚合酶链反应（PCR）技术，鉴定了金鱼促生长素（preproghrelin）的互补脱氧核糖核酸（cDNA）。全长490 bp的cDNA编码一个含有103个氨基酸的前促生长素，包含26个氨基酸的信号区、19个氨基酸的成熟肽区和55个氨基酸的c端肽区。金鱼胃饥饿素的成熟肽区有两个可能的裂解位点和酰胺化信号（GRR）；一个是12个氨基酸，另一个是19个氨基酸。在胃饥饿素“活性核心”的第二个氨基酸位置的丝氨酸(S)被苏氨酸(T)取代。金鱼的胃饥饿素基因有四个外显子和三个短内含子，类似于人类的胃饥饿素基因。RT-PCR法检测大鼠脑、垂体、肠、肝、脾和鳃组织中Ghrelin信使RNA （mRNA）的表达，Southern blot法检测小肠组织中Ghrelin信使RNA的表达。脑室（ICV）内注射正辛烷酰化金鱼胃饥饿素（1-19）刺激金鱼的食物摄入。
Complementary deoxyribonucleic acid (cDNA) encoding goldfish preproghrelin was identified using rapid amplification of the cDNA ends (RACE) and reverse transcription (RT)-polymerase chain reaction (PCR). The 490 bp cDNA encodes a 103 amino acid preproghrelin which has a 26 amino acid signal region, 19 amino acid mature peptide and a 55 amino acid C-terminal peptide region. The mature peptide region of goldfish ghrelin has two putative cleavage sites and amidation signals (GRR); one after 12 amino acids and the other after 19 amino acids. The serine (S) in the second amino acid position in the "active core" of ghrelin is substituted with threonine (T). The goldfish ghrelin gene has four exons and three short introns and resembles the human ghrelin gene. Ghrelin messenger RNA (mRNA) expression was detected in the brain, pituitary, intestine, liver, spleen and gill by RT-PCR followed by Southern blot analysis, and in the intestine by Northern blot. Intracerebroventricular (ICV) injection of n-octanoylated goldfish ghrelin (1-19) stimulates food intake in goldfish.