Expression of pTα mRNA in a committed dendritic cell precursor in the human thymus
Expression of pTα mRNA in a committed dendritic cell precursor in the human thymus
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DOI：
10.1182/blood.v94.8.2647.420k06_2647_2657
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发表时间：
1999-10-15
期刊：
影响因子：
20.3
通讯作者：
Spits, H
中科院分区：
文献类型：
--
作者：
Res, PCM;Couwenberg, F;Spits, H
我们已经鉴定了人类胸腺中的树突状细胞前体(Pre-DC)。这些CD1a(-)CD3(-)CD4(+)CD8(-)细胞在膜上表达高水平的IL-3Rα(IL-3Rα)，经IL-3和CD40结扎培养后能够发育为成熟的DC。DC前体主要位于胸腺髓质。有趣的是，前DC表达PTαmRNA，它也存在于CD1a(+)CD3(-)CD4(+)CD8(-)前T细胞中。然而，在适当的检测中，DC前-JACK表达重组激活基因-1mRNA并未发育成T细胞。胸腺前树突状细胞与最近在扁桃体T细胞区发现的树突前树突状细胞非常相似，提示这些前树突状细胞群体来自淋巴样起源。(C)1999年由美国血液病学会主办。
We have characterized dendritic cell precursors (pre-DC) in the human thymus. These CD1a(-)CD3(-)CD4(+)CD8(-) cells express high levels of interleukin-3R alpha (IL-3R alpha) on the membrane and are able to develop into mature DC upon culture with IL-3 and CD40 ligation. The DC precursors are predominantly located in the thymic medulla. Interestingly, the pre-DC express pT alpha mRNA, which is also present in CD1a(+)CD3(-)CD4(+) CD8(-) pre-T cells. Yet, the pre-DC jack expression of recombination activating gene-1 mRNA and fail to develop into T cells in appropriate assays. The thymic pre-DC are very similar to the recently characterized pre-DC found in the T cell areas of the tonsil, and it is suggested that these pre-DC populations are of lymphoid origin. (C) 1999 by The American Society of Hematology.