Expression and localization of gonadotropic hormone subunits (Gpa, Fshb, and Lhb) in the pituitary during gonadal differentiation in medaka
Expression and localization of gonadotropic hormone subunits (Gpa, Fshb, and Lhb) in the pituitary during gonadal differentiation in medaka
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DOI：
10.1016/j.ygcen.2014.04.036
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发表时间：
2014-08-01
影响因子：
2.7
通讯作者：
Kobayashi, Tohru
中科院分区：
文献类型：
--
作者：
Horie, Yoshifumi;Shimizu, Akio;Kobayashi, Tohru
为了阐明两种不同的促性腺激素(GTH)细胞的出现和时间变化，我们详细研究了青蛙d-RR品系在性腺分化和发育过程中GTH细胞的动态。性别决定基因DMY在受精后5天(DPF)的性腺体细胞中明显表达。无论遗传性别如何，糖蛋白-α(GPA)阳性细胞最早出现在第4天，而卵泡刺激素-β(FSHB)阳性细胞分别在第5天和第6天出现在XX和XY胚胎中。相比之下，青竹在孵化后70天(DPH)，雌雄之间均可观察到促黄体激素-β(LHB)阳性细胞。在0DPH时，XX仔鱼脑垂体FSHB阳性细胞密度显著高于XY仔鱼，此后在性成熟前无显著差异。在本研究中，观察到FSHB的时间表达，表明FSH细胞在孵化前开始分化，FSH细胞中的性别二型性在青竹性别决定后短暂地发生。(C)2014 Elsevier Inc.保留所有权利。
To clarify the appearance of and chronological changes in two different gonadotropic hormone (Gth) cells, we examined the dynamics of Gth cells in detail during gonadal differentiation and development in the d-rR strain of medaka (Olyzias latipes). Expression of the sex-determining gene Dmy was evident in gonadal somatic cells at 5 days post-fertilization (dpf). Glycoprotein-alpha (Gpa)-positive cells first appeared in the pituitary at 4 dpf, regardless of genetic sex, while follicle-stimulating hormone-beta (Fshb)-positive cells was detected in XX and XY embryos at 5 and 6 dpf, respectively. In contrast, luteinizing hormone-beta (Lhb)-positive cells were observed in both sexes of medaka after 70 days post-hatching (dph). The density of Fshb-positive cells in the pituitary was significantly and transiently higher in XX than in XY fry at 0 dph, and thereafter no significant differences were detected before sexual maturation. In this study, temporal expression of Fshb was observed, indicating that Fsh cells become differentiated before hatching and that sexual dimorphism in Fsh cells occurs transiently after sex determination in medaka. (C) 2014 Elsevier Inc. All rights reserved.