Neuronal labeling in the rat brain and spinal cord from the ovary using viral transneuronal tracing technique
Neuronal labeling in the rat brain and spinal cord from the ovary using viral transneuronal tracing technique
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DOI：
10.1159/000054372
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发表时间：
1998-10-01
影响因子：
4.1
通讯作者：
Halasz, B
中科院分区：
文献类型：
--
作者：
Gerendai, I;Toth, IE;Halasz, B
在目前的调查中，病毒transneuronal标记方法，这是能够揭示中枢神经系统（CNS）神经元的层次链，被施加到确定在中枢神经系统中的网站与卵巢连接，并推测参与卵巢功能的控制。将伪狂犬病病毒注射到大鼠的卵巢中，几天后（注射后的不同时间），检查脊髓和大脑中是否有来自卵巢的病毒感染的神经元。用多克隆抗病毒抗体对病毒标记的神经细胞进行免疫细胞化学鉴定。在脊髓和脑中都检测到病毒标记的神经元。在脊髓中，这些元素被观察到在中间外侧细胞柱，在背角接近边缘区和中央自主神经核。在延髓和围栏中，发现几个核和细胞群（最后区，孤束核，迷走神经背侧复合体，疑核，巨细胞旁核，锥体旁核，A1，A5和A7细胞群，中缝尾侧核，蓝斑，蓝斑下核，Barrington核，Kolliker-Kolliker核）的神经元被跨神经元标记。在中脑，中脑导水管周围灰质的腹外侧部分含有病毒标记的神经元。在间脑，下丘脑室旁核内可见非常密集的胞体标记，下丘脑外侧核、背侧核、弓状核、齿状回、穹窿周围区和下丘脑前部也可见少量病毒感染神经元。关于端脑结构，在终纹的床核和中央杏仁核中发现病毒标记细胞。这些研究结果提供了第一个神经形态学证据卵巢和中枢神经系统之间的多突触神经元通路的存在，并给出了详细的说明，在这条通路所涉及的结构。
In the present investigations the viral transneuronal labeling method, which is able to reveal hierarchial chains of central nervous system (CNS) neurons, was applied to identify sites in the CNS connected with the ovary and presumably involved in the control of ovarian functions. Pseudorabies virus was injected into the ovaries of rats and a few days later (at various times after the injection) the spinal cord and brain were examined for virus-infected neurons from the ovary. The virus-labeled nerve cells were identified by immunocytochemistry using polyclonal antiviral antibody. Virus-labeled neurons were detected both in the spinal cord and the brain. In the spinal cord such elements were observed in the intermediolateral cell column, in the dorsal horn close to the marginal zone and in the central autonomic nucleus. In the medulla oblongata and pens, neurons of several nuclei and cell groups (area postrema, nucleus of the solitary tract, dorsal vagal complex, nucleus ambiguus, paragigantocellular nucleus, parapyramidal nucleus, A1, A5 and A7 cell groups, caudal raphe nuclei, locus ceruleus, subceruleus nucleus, Barrington's nucleus, Kolliker-Fuse nucleus) were found to be transneuronally labeled. In the mesencephalon, the ventrolateral part of the periaqueductal gray matter contained virus-labeled neurons. In the diencephalon, a very intensive cell body labeling was observed in the hypothalamic paraventricular nucleus and a few virus-infected neurons could be detected in the lateral and dorsal hypothalamus, in the arcuate nucleus, zona incerta, perifornical area and in the anterior hypothalamus. Concerning the telencephalic structures, virus-labeled cells were found in the bed nucleus of the stria terminalis and in the central amygdala nucleus. These findings provide the first neuromorphological evidence for the existence of a multisynaptic neuronal pathway between the ovary and the CNS, and give a detailed account of the structures involved in this pathway.