PERSISTENCE OF INTRINSIC NEURONS AND POSSIBLE PHENOTYPIC CHANGES AFTER EXTRINSIC DENERVATION OF HUMAN RESPIRATORY-TRACT BY HEART-LUNG TRANSPLANTATION
PERSISTENCE OF INTRINSIC NEURONS AND POSSIBLE PHENOTYPIC CHANGES AFTER EXTRINSIC DENERVATION OF HUMAN RESPIRATORY-TRACT BY HEART-LUNG TRANSPLANTATION
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DOI：
10.1164/ajrccm/141.6.1538
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发表时间：
1990-06-01
期刊：
影响因子：
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通讯作者：
YACOUB, MH
中科院分区：
文献类型：
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作者：
SPRINGALL, DR;POLAK, JM;YACOUB, MH
呼吸道神经具有器官外部(感觉、交感)和内部(副交感)的细胞体，并含有生物活性多肽。包括降钙素基因相关肽和速激肽(感觉神经)、血管活性肠多肽(副交感神经)和含有酪氨酸的神经肽(交感神经)。由于移植中断了外源性神经对组织的供应，我们检查了在初次移植后2至42个月再次移植时取出的移植的人呼吸道(n=11)，以确定是否有神经和多肽合成持续存在。作为建立人类呼吸道神经分布的对照，取自10例肺切除和5例身体解剖组织。冰冻切片免疫组织化学染色显示神经标记物PGP9.5、神经肽和儿茶酚胺合成酶酪氨酸羟基酶。所有移植组织均检测到PGP 9.5免疫反应阳性神经。它们的数量总体上比对照组织少，在气管和支气管上皮中明显如此，只有3例病例中它们稀少。移植神经中酪氨酸羟化酶免疫反应明显减少。除血管活性肠多肽仅在肺血管内有明显变化外，移植体内的多肽免疫反应神经的数量也减少。5例移植组织中可见神经节细胞酪氨酸羟化酶和酪氨酸神经肽免疫反应阳性，而对照组织中未见。我们的结论是，尽管一些神经和神经肽的合成在外源性肺去神经后仍然存在，但也发生了潜在的显著变化，包括在内在副交感神经元中出现儿茶酚胺合成酶的免疫反应，以及交感神经中通常存在的一种肽。
Respiratory tract nerves have cell bodies outside (sensory, sympathetic) and inside (parasympathetic) the organ and contain bioactive peptides. These included calcitonin gene-related peptide and tachykinins (sensory nerves), vasoactive intestinal polypeptide (parasympathetic nerves), and neuropeptide with tyrosine (sympathetic nerves). Because transplantation interrupts the extrinsic nerve supply to the tissues, we have examined transplanted human respiratory tracts (n = 11) removed at retransplantation 2 to 42 months after the primary transplant in order to determine whether any nerves and peptide synthesis persist. As controls to establish nerve distribution in human respiratory tract, tissues were obtained from 10 lung resections and five autopsies. Cryostat sections were immunostained to demonstrate the general neural marker PGP 9.5, neuropeptides, and the catecholamine-synthesizing enzyme tyrosins hydroxylase. Nerves immunoreactive for PGP 9.5 were detected in all transplanted tissues. They were fewer in number overall than in control tissue, significantly so in epithelium of trachea and bronchus where they were present sparsely in only three cases. Nerves immunoreactive for tyrosine hydroxylase were significantly fewer in the transplants. Peptide-immunoreactive nerves were also reduced in number in the transplants, except for vasoactive intestinal polypeptide, which was only significantly changed in blood vessels in the lung. Ganglion cells immunoreactive for tyrosine hydroxylase and neuropeptide with tyrosine were seen in the transplanted tissues in five cases, but never in the control tissues. We conclude that whereas some nerves and neuropeptide synthesis persist after extrinsic pulmonary denervation, potentially significant changes also occur, including the appearance in intrinsic parasympathetic neurones of immunoreactivity for a catecholamine-synthesizing enzyme and a peptide normally found in sympathetic nerves.