Adrenergic antagonists, and a possible link between the increase in cyclic adenosine 3′,5′‐monophosphate and DNA synthesis during liver regeneration
Adrenergic antagonists, and a possible link between the increase in cyclic adenosine 3′,5′‐monophosphate and DNA synthesis during liver regeneration
复制标题
肾上腺素能拮抗剂，以及肝再生过程中环腺苷 3′,5′-单磷酸增加与 DNA 合成之间的可能联系
DOI：
10.1002/jcp.1040820204
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发表时间：
1973
影响因子：
5.6
通讯作者：
J. Whitfield
中科院分区：
文献类型：
--
作者：
J. Macmanus;B. Braceland;T. Youdale;J. Whitfield
70%肝切除术后，肝残体中的cAMP浓度以双相方式增加，在3和12小时达到峰值，DNA合成在18小时开始。在手术后30分钟注射普萘洛尔（dl）停止了第一波环AMP蓄积，但不影响第二波蓄积或DNA合成的启动。然而，在8小时注射dl，普萘洛尔同样延迟（6至8小时）环AMP积累的第二波和DNA合成的开始。普萘洛尔（dl）本身并不影响DNA复制，因为在第二波环AMP积累结束后，DNA合成开始后，普萘洛尔完全无效。普萘洛尔（dl）的作用不是由于阻断β-肾上腺素能受体，因为其d或l异构体单独无作用，与不相关的β-肾上腺素能受体阻滞剂一样（Kü 1313和M&B 17 - 803 A）。
The cyclic‐AMP concentration in the liver remnant after 70% hepatectomy increases in a biphasic manner with peak values at 3 and 12 hours, and DNA synthesis begins at 18 hours. Propranolol (dl) injected at 30 minutes after surgery stopped the first wave of cyclic‐AMP accumulation, but did not affect the second accumulation or the initiation of DNA synthesis. However, dl, propranolol injected at eight hours equally delayed (by 6 to 8 hours) the second wave of cyclic‐AMP accumulation and the initiation of DNA synthesis. Propranolol (dl) did not affect DNA replication per se, since it was totally ineffective after the second wave of cyclic‐AMP accumulation had passed and DNA synthesis had been initiated. Propranolol (dl) action was not due to a blockade of β‐adrenergic receptors, since its d or l isomers were separately without effect, as were unrelated β‐adrenergic blockers (Kü 1313 and M&B 17‐803A).