LUMEN FORMATION BY EPITHELIAL-CELL LINES IN RESPONSE TO COLLAGEN OVERLAY - A MORPHOGENETIC MODEL IN CULTURE
LUMEN FORMATION BY EPITHELIAL-CELL LINES IN RESPONSE TO COLLAGEN OVERLAY - A MORPHOGENETIC MODEL IN CULTURE
复制标题
DOI：
10.1073/pnas.79.15.4672
复制
发表时间：
1982-01-01
期刊：
影响因子：
--
通讯作者：
BISSELL, MJ
中科院分区：
文献类型：
--
作者：
HALL, HG;FARSON, DA;BISSELL, MJ
两种细胞系，Madin-Darby犬肾（MDCK）和正常鼠乳腺（NMuMG），在胶原凝胶上生长为单层，用另一种胶原凝胶覆盖。细胞通过重组对覆盖物作出反应，从而产生管腔。MDCK细胞形成的管腔合并形成与小管大小相当的大空腔。NMuMG细胞在小管腔周围形成簇，类似于腺泡。通过光学显微镜和EM在此描述的特征性排列类似于细胞系来源的组织的形态。MDCK细胞，生长在血清的存在下，形成管腔是否血清被删除时的覆盖，NMuMG细胞需要一个不可透析的成分的血清或激素补充剂在无血清的限定培养基。在谷氨酰胺类似物6-重氮-5-氧代-L-正亮氨酸存在下，MDCK细胞延迟管腔形成，但该化合物不影响NMuMG管腔形成。在这两种细胞系中，管腔形成不受硫酸盐缺乏、硫酸化糖胺聚糖合成抑制剂或胶原蛋白合成抑制剂存在的影响。DNA合成伴随管腔形成，但不是必需的。
Two cell lines, Madin-Darby canine kidney (MDCK) and normal murine mammary gland (NMuMG), growing as monolayers on collagen gels were overlaid with another collagen gel. The cells responded to the overlay by undergoing reorganization resulting in the creation of lumina. MDCK cells formed lumina that coalesced to form large cavities comparable in size with a tubule. NMuMG cells formed clusters surrounding small lumina, which appeared similar to acini of glandular tissue. The characteristic arrangements, described here by light microscopy and EM, resembled the morphology of the tissues of cell line origin. MDCK cells, grown in the presence of serum, formed lumina whether or not serum was removed at the time of overlay, NMuMG cells required either a nondialyzable component of serum or hormonal supplements in serum-free defined media. Lumen formation was delayed by MDCK cells in the presence of the glutamine analog 6-diazo-5-oxo-L-norleucine, but this compound did not affect NMuMG lumen formation. In both cell lines, lumen formation was unaffected by the absence of sulfate, the presence of an inhibitor of sulfated glycosaminoglycan synthesis or an inhibitor of collagen synthesis. DNA synthesis accompanied lumen formation but was not required.