A developmentally regulated switch from stem cells to dedifferentiation for limb muscle regeneration in newts.
A developmentally regulated switch from stem cells to dedifferentiation for limb muscle regeneration in newts.
复制标题
从干细胞到肢体肌肉再生的开发调节转换。
DOI：
10.1038/ncomms11069
复制
发表时间：
2016-03-30
影响因子：
16.6
通讯作者：
Chiba C
中科院分区：
文献类型：
--
作者：
Tanaka HV;Ng NCY;Yang Yu Z;Casco-Robles MM;Maruo F;Tsonis PA;Chiba C
蝾螈是一种有尾目两栖动物，在其一生中能够反复再生其四肢，而其他两栖动物在变态后退化或失去再生四肢的能力。蝾螈的这种特殊能力是否归因于一种控制幼虫再生的策略，或者是蝾螈在变态后发明的一种新策略，还有待确定。在这里，我们报告说，蝾螈切换肢体再生的细胞机制从干/祖细胞为基础的机制（幼虫模式）的去分化为基础的（成人模式），因为它超越变态。我们表明，幼虫蝾螈使用干/祖细胞，如卫星细胞的新肌肉再生肢体，而变态蝾螈招募肌纤维细胞在树桩为同一目的。我们的结论是，蝾螈已经发展出新的策略，以确保其再生能力的四肢变态后。 蝾螈的肢体再生是如何在细胞水平上被调节的，这是一个很有争议的问题。在这里，作者展示了在不同发育阶段起作用的不同机制，即幼虫再生中的干/祖细胞和芽基中的肌纤维调节变态后的肢体再生。
The newt, a urodele amphibian, is able to repeatedly regenerate its limbs throughout its lifespan, whereas other amphibians deteriorate or lose their ability to regenerate limbs after metamorphosis. It remains to be determined whether such an exceptional ability of the newt is either attributed to a strategy, which controls regeneration in larvae, or on a novel one invented by the newt after metamorphosis. Here we report that the newt switches the cellular mechanism for limb regeneration from a stem/progenitor-based mechanism (larval mode) to a dedifferentiation-based one (adult mode) as it transits beyond metamorphosis. We demonstrate that larval newts use stem/progenitor cells such as satellite cells for new muscle in a regenerated limb, whereas metamorphosed newts recruit muscle fibre cells in the stump for the same purpose. We conclude that the newt has evolved novel strategies to secure its regenerative ability of the limbs after metamorphosis. How limb regeneration in the newt is regulated at a cellular level is much debated. Here, the authors show different mechanisms acting at different developmental stages, namely stem/progenitor cells in larval regeneration and muscle fibres in the blastema regulate limb regeneration after metamorphosis.