The cis-acting RNA trafficking signal from myelin basic protein mRNA and its cognate trans-acting ligand hnRNP A2 enhance cap-dependent translation.
The cis-acting RNA trafficking signal from myelin basic protein mRNA and its cognate trans-acting ligand hnRNP A2 enhance cap-dependent translation.
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DOI：
10.1083/jcb.147.2.247
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发表时间：
1999-10-18
影响因子：
7.8
通讯作者：
Carson, J H
中科院分区：
文献类型：
--
作者：
Kwon, S;Barbarese, E;Carson, J H
21 核苷酸 RNA 运输信号 (RTS) 最初在髓磷脂碱性蛋白 mRNA 中发现，但也在多种其他局部 RNA 中发现，对于少突胶质细胞中沿着微管运输 RNA 来说是必要且充分的。 RTS 特异性结合 RNA 结合蛋白 hnRNP A2。 RTS 和 hnRNP A2 共同构成 RNA 运输途径中多个步骤的顺式/反式决定簇。在这里，我们表明，将 RTS 插入绿色荧光蛋白 (GFP) RNA 中可以增强翻译，而不影响显微注射 RNA 的稳定性。在双顺反子 RNA 中，RTS 增强帽依赖性翻译，而不影响内部核糖体进入位点 (IRES) 依赖性翻译。 RTS 的翻译增强子功能与位置、拷贝数和细胞类型无关，依赖于 hnRNP A2，并且随着注射 RNA 量的增加而饱和。这代表了在哺乳动物系统中鉴定的第一个特异性翻译增强子元件之一。
The 21 nucleotide RNA trafficking signal (RTS), originally identified in myelin basic protein mRNA, but also found in a variety of other localized RNAs, is necessary and sufficient for transport of RNA along microtubules in oligodendrocytes. The RTS binds specifically to the RNA binding protein, hnRNP A2. Together, the RTS and hnRNP A2 comprise cis/trans determinants for several steps in the RNA trafficking pathway. Here we show that insertion of the RTS into green fluorescent protein (GFP) RNA enhances translation without affecting stability of microinjected RNA. In dicistronic RNA, the RTS enhances cap-dependent translation without affecting internal ribosome entry site (IRES)-dependent translation. The translation enhancer function of the RTS is position, copy number, and cell type independent, hnRNP A2 dependent, and saturable with increasing amounts of injected RNA. This represents one of the first specific translation enhancer elements identified in a mammalian system.