Comparative Genomics Analysis of the Aromatic and Xenobiotic Degradation Capacities and Heavy Metal Resistance in Seven Environmentally Derived Bacterial Isolates
Comparative Genomics Analysis of the Aromatic and Xenobiotic Degradation Capacities and Heavy Metal Resistance in Seven Environmentally Derived Bacterial Isolates
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DOI：
10.1007/s11270-023-06495-2
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发表时间：
2023-07
期刊：
影响因子：
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通讯作者：
A. Kneubehl;Rupa Iyer
中科院分区：
文献类型：
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作者：
A. Kneubehl;Rupa Iyer
这项工作是对我们实验室先前鉴定的环境细菌分离株（木糖氧化无色杆菌 ADAF13、Exiguobacteria sp.）的芳香族和外源化合物降解能力以及重金属耐受性进行的比较基因组学研究。 KKBO11、人苍白杆菌FRAF13、恶臭假单胞菌CBF10-2、施氏假单胞菌ODKF13、放射根瘤菌GHKF11 和嗜麦芽寡养单胞菌CBF10-1。这项工作旨在评估这些分离株作为生物修复工具的潜力。我们发现了多种芳香族降解途径，但没有直接作用于工业化合物，如多环芳香族化合物、苯、邻苯二甲酸盐或二甲苯。木糖氧化无色杆菌ADAF13，P。恶臭CBF10-2，和P。 StutzeriODKF13 显示了最完整的芳香族化合物降解和卤代苯甲酸酯降解途径。所有分离株均含有砷、镉、铜、铬、铅、汞和锌的重金属抗性基因。砷抗性基因在分离株中最常见，并被组织成结构多样的砷操纵子。总的来说，我们的数据表明A.木糖氧化酶ADAF13，P。恶臭CBF10-2，和P。 StutzeriODKF13 是进一步增强和开发生物修复工具的有力候选者。
This work is a comparative genomics investigation of the aromatic and xenobiotic compound degradation capabilities and heavy metal resistance of environmental bacterial isolates previously identified by our lab,Achromobacter xylosoxidansADAF13,Exiguobacteriumsp. KKBO11,Ochrobactrum anthropiFRAF13,Pseudomonas putidaCBF10-2,Pseudomonas stutzeriODKF13,Rhizobium radiobacterGHKF11, andStenotrophomonas maltophiliaCBF10-1. This work sought to assess the potential of these isolates as bioremediation tools. We found a variety of aromatic degradation pathways though none directly acts on industrial compounds such as polycyclic aromatic compounds, benzene, phthalate, or xylene.Achromobacter xylosoxidansADAF13,P. putidaCBF10-2, andP. stutzeriODKF13 showed the most complete pathways for aromatic compound degradation and halobenzoate degradation. All isolates contained heavy metal resistance genes for arsenic, cadmium, copper, chromium, lead, mercury, and zinc. Arsenic resistance genes were the most common among isolates and were organized into structurally diversearsoperons. Collectively, our data indicated thatA. xylosoxidansADAF13,P. putidaCBF10-2, andP. stutzeriODKF13 are strong candidates for further enhancement and development as bioremediation tools.